Biodiesel is an attractive alternative fuel because of its nontoxicity and biodegradability properties. Biodiesel is produced through transesterification of vegetable oils’ triglyceride. It is obtained from vegetable oils or fats either by chemical or enzyme-catalyzed transesterification with methanol or ethanol. By using whole-cell biocatalyst immobilized within biomass support particles (BSPs...

This work demonstrates the first example of the immobilisation of MAO-N whole cells to produce a biocatalyst that remained suitable for repetitive use after 11 months of storage and stable up to 15 months after immobilisation. The production ofEscherichia coli expressing recombinant MAON was scaled up to bioreactors under regulated, previously optimised conditions (10 % DO, pH 7), and the amoun...

Enzymatic mode of biodiesel production has recently gained a special attention among researchers because it eliminates the disadvantages of alkaline process such as end product recovery, waste water treatment, energy intensive and etc. Recently, considerable attention has paid in the direct use of intracellular lipase as a whole-cell biocatalyst (i.e immobilization of the enzyme secreting micro...

BACKGROUND Biocatalyst improvement through molecular and recombinant means should be complemented with efficient process design to facilitate process feasibility and improve process economics. This study focused on understanding the bioprocess limitations to identify factors that impact the expression of the terminal hydroxylase CYP153A6 and also influence the biocatalytic transformation of n-o...

The C-C bond formation is an important reaction in organic synthesis to obtain value-added intermediates. Therefore, in this paper an attempt has been made to accelerate the Henry reaction (C-C bond formation) between aryl aldehydes and nitromethane using less expensive whole cell biocatalyst, baker’s yeast (BY). The scope of the methodology was also tested for the heteryl aldehyde i.e. 2-chlor...

Although the routes of de novo pyridoxal 5'-phosphate (PLP) biosynthesis have been well described, studies of the engineering of an intracellular PLP supply are limited, and the effects of cellular PLP levels on PLP-dependent enzyme-based whole-cell biocatalyst activity have not been described. To investigate the effects of PLP cofactor availability on whole-cell biocatalysis, the ribose 5-phos...

Short-chain aliphatic esters are small volatile molecules that produce fruity and pleasant aromas and flavors. Most of these esters are artificially produced or extracted from natural sources at high cost. It is, however, possible to 'naturally' produce these molecules using biocatalysts such as lipases and esterases. A gene coding for a newly uncovered lipase was isolated from a previous metag...