it is well accepted that in experimental model of leishmania major infection, balb/c mice mount a th2 response and produce igg1 predominantly whereas c57bl/6mice enhance th1 response with the biased production of igg2a antibodies. therefore, screening for parasite antigens on the basis of reactivity with sera from infected susceptible or resistant mice might be used for the identification of th...

Western Blot tekniği, herhangi bir örnekten istenen tek proteini özgün şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik izoformlarının belirlenmesinde, genetik hastalıkların ortaya çıkışından sorumlu prot...

background and aims: hiv is spreading rapidly among people word wide. infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome (aids).early hiv detection is depended on antibody screening against virus by enzyme linked immunosorbent assay (elisa).some confirmatory tests such as western blot and recombinant immunobloting assay (riba), are used to veri...

serological methods are commonly used methods for detection of viruses. preparation of pure viral antigens is a crucial step in production of antibodies required for serological studies. in this research the gene encoding coat protein of a beet western yellows virus (bwyv) isolate from iran was amplified by pcr and was ligated into a bacterial expression vector (pet26b) to obtain pet-bwyv-cp cl...

citrus tristeza virus (ctv) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in iran, as one of the major citr...

cystic echinococcosis (ce) is one of the most important parasitic zoonosis worldwide. due to the high recurrence rate of the disease after surgery, follow up of the patient is necessary. the aim of current research was to assess the performance of western blotting (wb), using sheep hydatid fluid, for serological diagnosis and post-treatment monitoring of human ce.serum samples obtained from 50 ...

background : ascaridia galli , the largest nematode of small intestine of birds, especially the native poultry, may give rise to serious illness, pathological defects and economical losses even in modern poultry production systems. although various measures have been undertaken to vaccinate poultry against a.galli , no satisfactory results were obtained so far. however, there is no report on th...

partially fed (4 -5 days) h .a. anatolicum (iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (gs), gut membrane antigen (gm), triton extracted gut membrane antigen ( tx-gm ) and sodium dodecyl sulphate treated triton extracted gut membrane antigen (sds-tx-gm). the new zealand white rabbits were immunized with gs, gm, tx- gm...

in leishmania (l.) major-infected balb/c mice th2-type cells results in disease progression, whereas c57bl/6-infected mice mount a th1-type response, which leads to control of the infection. th2 response correlates with igg1 whereas, th1 response supports switching to igg2a subclasses. since igg isotype-dominated response depends on different cd4 + t cell subsets, we studied the antigenic profi...

umbelliprenin is a prenylated compound, which belongs to the class of sesquiterpene coumarins. it is extracted from dried roots of ferula szwitsiana collected from the mountains of golestan forest (golestan province, north of iran). induction of apoptosis in jurkat t-cll cells has been previously shown. in this study, effect of umbelliprenin on proapoptotic caspases (caspase-8 and -9) and antia...