نتایج جستجو برای: Watermelon silver mottle virus

تعداد نتایج: 444594  

Journal: :iranian journal of biotechnology 2015
xueqin rao jie sun

background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...

2013
Ratthaphol Charlermroj Orawan Himananto Channarong Seepiban Mallika Kumpoosiri Nuchnard Warin Michalina Oplatowska Oraprapai Gajanandana Irene R. Grant Nitsara Karoonuthaisiri Christopher T. Elliott

Plant pathogens are a serious problem for seed export, plant disease control and plant quarantine. Rapid and accurate screening tests are urgently required to protect and prevent plant diseases spreading worldwide. A novel multiplex detection method was developed based on microsphere immunoassays to simultaneously detect four important plant pathogens: a fruit blotch bacterium Acidovorax avenae...

2014
Wei-Te Chen Chien-Hao Tseng Chi-Wei Tsai

Thrips-borne tospoviruses cause numerous plant diseases that produce severe economic losses worldwide. In the disease system, thrips not only damage plants through feeding but also transmit causative agents of epidemics. In addition, thrips are infected with tospoviruses in the course of virus transmission. Most studies on the effect of tospoviruses on vector thrips have focused on the Tomato s...

Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

Journal: :Iranian journal of biotechnology 2015
Xueqin Rao Jie Sun

BACKGROUND Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. OBJECTIVES Development of a highly sensitive and reliable detection method for WSMoV. MATERIALS AND METHODS Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...

2015
Chung-Hao Huang Weng-Rong Hsiao Ching-Wen Huang Kuan-Chun Chen Shih-Shun Lin Tsung-Chi Chen Joseph A. J. Raja Hui-Wen Wu Shyi-Dong Yeh Tetsuro Ikegami

The NSs protein of Watermelon silver mottle virus (WSMoV) is the RNA silencing suppressor and pathogenicity determinant. In this study, serial deletion and point-mutation mutagenesis of conserved regions (CR) of NSs protein were performed, and the silencing suppression function was analyzed through agroinfiltration in Nicotiana benthamiana plants. We found two amino acid (aa) residues, H113 and...

2017
Nam-Gyu Kim Eun-Young Seo Sang-Hyuk Han Jun-Su Gong Cheol-Nam Park Ho-Seop Park Leslie L Domier John Hammond Hyoun-Sub Lim

Efforts to control viral diseases in crop production include several types of physical or chemical treatments; antiviral extracts of a number of plants have also been examined to inhibit plant viral infection. However, treatments utilizing naturally selected microorganisms with activity against plant viruses are poorly documented. Here we report isolation of a soil inhabiting bacterium, Pseudom...

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