The processing of cheese whey pre-concentrated by reverse osmosis is carried out through ultrafiltration in diafiltration mode to produce whey protein concentrates with lower lactose content to be incorporated in beverages. The initial cheese whey protein and lactose contents are 2.13g/100g and 13.22g/100g, respectively.The commercial membranes, GR95PP, supplied by Alfa Laval, Denmark, we...

Hypoallergenic antibacterial low-molecular-mass peptides were produced from defatted soybean meal in a membrane bioreactor. In the first step, soybean meal proteins were digested with trypsin in the bioreactor, operated in batch mode. For the tryptic digestion of soybean meal protein, optimum initial soybean meal concentration of 75 g/L, temperature of 40 °C and pH=9.0 were determined. After en...

Diafiltration is an ultrafiltration membrane technique for completely removing, replacing, or lowering the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids, and other biomolecules. The process selectively uses permeable (porous) membrane filters to separate the components of solutions and suspensions based on their molecular size. Smaller molecules ...

Diafiltration is term used reflecting tangential flow filtration (TFF) method of “washing” or removing a permeable molecule (impurities, salts, solvents, small proteins, etc) from a solution in an accelerated method when compared to dialysis tubing. By selecting the appropriate membrane(s), it can also be used to exchange buffers, narrow particle size distributions, modify salt concentrations o...

Looking at current trends within downstream processing (DSP) of high value bioproducts, it shows that there are ongoing efforts in replacing batch processes by continuous variants. However, a unit procedure which still lacks simple and compact variant is diafiltration. Here, we present such single piece diafiltration equipment achieving buffer exchange up to 99.90%. The device composed 3D-print...

The direct utilisation of side streams from membrane processing and evaporation is great potential interest for dairies to reduce water consumption. In an analytical screening 89 industrially obtained streams, samples varied in pH conductivity broad ranges 4.22 7.56 0.001 0.949 Siemens per meter, respectively. Detailed compositional data the enabled assessment their applicability as diafiltrati...