This study investigated the interaction between eupatorin and bovine serum albumin (BSA) using ultraviolet-visible (UV-vis) absorption, fluorescence, synchronous fluorescence, circular dichroism (CD) spectroscopies, and molecular modeling at pH 7.4. Results of UV-vis and fluorescence spectroscopies illustrated that BSA fluorescence was quenched by eupatorin via a static quenching mechanism. The...

Objective: The present study is designed to investigate the interaction of titanium dioxide nanoparticle (TiO2NPs) with human serum albumin (HSA) using spectroscopic techniques. Methods: TiO2NPs was characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy. Effect of NPs on the conformation of HSA was evaluated by ...

the aggregation behavior of anionic cobalt(ii) 4,4′,4ʺ,4‴-tetrasulfonated phthalocyanine, [co(tspc)4-] was studied at its various concentrations and different ionic strengths using optical absorption and resonance light scattering (rls) spectroscopies in 5 mm phosphate buffer, ph 7.0 at 25 °c. the results show no aggregation behavior at concentration range of 5.1 × 10-6-7.5 × 10-5 m. also, the ...

The aggregation behavior of anionic Cobalt(II) 4,4′,4ʺ,4‴-tetrasulfonated phthalocyanine, [Co(TSPc)4-] was studied at its various concentrations and different ionic strengths using optical absorption and resonance light scattering (RLS) spectroscopies in 5 mM phosphate buffer, pH 7.0 at 25 °C. The results show no aggregation behavior at concentration range of 5.1 × 10-6-7....

a novel podand based anion receptor bearing benzimidazole motifs as recognition sites was designed and synthesized by reaction of 1,7-bis(2'-methylbenzoate)-1,4,7-trioxaheptane and phenylenediamine. the binding properties of anionic guest with this receptor were studied using uv-vis and fluorescence spectroscopy. these studies revealed that this receptor exhibit selective recognition towards f- ...

Thermodynamics of the binding of antibiotic streptomycin to bovine serum albumin have been studied using isothermal titration calorimetry in combination with fluorescence, UV–vis and circular dichroism spectroscopies. The values of van’t Hoff enthalpy calculated from the temperature dependence of the binding constant do not agree with the calorimetric enthalpies indicating temperature dependent...

Puerarin, an isoflavone glycoside extracted from Pueraria plants, has various medical functions. Cytochrome P450s (CYPs) are crucial phase I metabolizing enzymes, which have been spotlighted for their effects on drug metabolism. The interaction between puerarin and CYPs (CYP1A2 and CYP2D6) was investigated by fluorescence, UV-Vis and circular dichroism spectroscopies, as well as molecular docki...

The surface parameter of nanoparticles such as hydrophobicity and a hydrophilicity on protein structure and function is very important. In this study, conformational changes of glucose oxidase (GOx) in the mercaptopurine: GNPs and 11-mercaptoundecanoic acid: GNPs as a hydrophobic and a hydrophilic GNPs surface was investigated by various spectroscopic techniques, including: UV-Vis absorption, f...

The surface parameter of nanoparticles such as hydrophobicity and a hydrophilicity on protein structure and function is very important. In this study, conformational changes of glucose oxidase (GOx) in the mercaptopurine: GNPs and 11-mercaptoundecanoic acid: GNPs as a hydrophobic and a hydrophilic GNPs surface was investigated by various spectroscopic techniques, including: UV-Vis absorption, f...

the surface parameter of nanoparticles such as hydrophobicity and a hydrophilicity on protein structure and function is very important. in this study, conformational changes of glucose oxidase (gox) in the mercaptopurine: gnps and 11-mercaptoundecanoic acid: gnps as a hydrophobic and a hydrophilic gnps surface was investigated by various spectroscopic techniques, including: uv-vis absorption, f...