Sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa's lesser uptake of exogenous DNA. In the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton X100 and DMSO, on exogenous DNA uptake by sheep spermatozoa and...

sperm mediated gene transfer can be an inexpensive and simple method in animal transgenesis; however its efficiency is poor, mainly due to the spermatozoa’s lesser uptake of exogenous dna. in the present study, the effects of lipofection and other augmentation techniques, such as sperm freezing and spermatozoa treatment with triton x100 and dmso, on exogenous dna uptake by sheep spermatozoa and...

lnillbition experiments with paraoxon revealed two types of acetylcholinesterases in Panagrellus redivivus i.e. a fast (F-component) and a slow reacting component (S-component). The F-component was thermostable and inactivated by Triton-X100, while the S-component was thermolabile and insensitive for Triton-X-100. The bimolecular rate constant for inhibition by paraoxon was 25.5 x 103 M-1min' f...

Ca2+-pump of sarcoplasmic reticulum was partially purified and solubilized with Triton-X100 in the presence of excess phospholipids. Vesicles were reconstituted by removal of Triton with Amberlite XAD-2 and the subsequent freeze-thaw/sonication method (Kasahara, M. and Hinkle, P. C. (1977) J. Biol. Chem., 252: 7384-7390). In the reconstituted system, H+ was ejected during Ca2+ uptake and in the...

In this research, electrodeposition procedure was utilized for the synthesis of a new composite polymer: N-methylpyrrole–Triton–X100/N, N,N-diethylaniline (NMPY-TRX100/NNDEA) used as coating on carbon steel type OL 37 electrode corrosion protection. The surfactant Triton–X100, dopant ion throughout process electropolymerization, had significant impact protection by impeding penetration corrosiv...

An important factor complicating the recovery of recombinant proteins from Escherichia coli is their intracellular location. An alternative to the commonly used method of releasing these proteins by mechanical disruption is to chemically permeabilize the cells. The objective of this research was to characterize the protein release kinetics of a permeabilization process using guanidine-HCl and T...

This research shows the development of a miniaturized solid-phase extraction method with UV-Vis detection for simultaneous determination dibenzofuran, fluoranthene and phenanthrene using chemometrics approaches. After synthesis Fe3O4 nanoparticles (Fe3O4 NPs), surface was modified by Triton X100 coating. The influence solvent volume, concentration X100, time, sample pH were studied optimized. D...