Trichinella larvae collected from wildlife, domestic and synanthropic animals in Finland were identified to species by two molecular techniques: Random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) and the recently described multiplex PCR. The RAPD-PCR was very sensitive to the sub-optimal preservation muscle larvae and resulting in weak and smeared bands on the gels for such...

Freezing of fox carcasses to minimize professional hazard of infection with Echinococcus multilocularis is recommended in endemic areas, but this could influence the detection of Trichinella larvae in the same host species. A method based on artificial digestion of frozen fox muscle, combined with larva isolation by a sequential sieving method (SSM), was validated using naturally infected foxes...

Cell-mediated immunity during the muscular phase of Trichinella infection in humans was studied. Cell proliferation, the phenotypic changes in the T-cell population, and expression and production of cytokines were examined by using peripheral blood mononuclear cells (PBMC) collected at different times postinfection from 10 individuals who had acquired Trichinella spiralis and five individuals w...

In order to study the epidemiology of trichinellosis with special emphasis on swine a detailed study was performed. The goals of the study were to define the prevalence of trichinellosis in swine and rats and to determine the etiological agents of the disease with special emphasis on sylvatic species, Trichinella britovi. Considering the obtained data it can be concluded that the risk of Trichi...

Muscle samples from 7,142 wild boars (Sus scrofa), 80 free-ranging hogs, 1,462 red foxes (Vulpes vulpes) 90 mustelids, 15 Alpine marmots (Marmota marmota), 873 rodents and 92 insectivores were examined for Trichinella Railliet, 1895. A wild boar, a badger (Meles meles) and 30 foxes (2.1%) were found to be infected. Eleven vulpine isolates and the wild boar isolate were identified as Trichinella...

BACKGROUND The objective of this investigation was to detect the presence of Trichinella in some carnivores of Mashhad in northeast of Iran and to identify Trichinella species circulating in this area. METHODS The present study was carried out using muscle tissue collected from 120 stray dogs, 26 wild boars, 25 rodents, two foxes and two hyenas captured in Mashhad City, province of Khorasan R...

b ackground: the objective of this investigation was to detect the presence of trichinella in some carnivores of mashhad in northeast of iran and to identify trichinella species circulating in this area. methods: the present study was carried out using muscle tissue collected from 120 stray dogs, 26 wild boars, 25 rodents, two foxes and two hyenas captured in mashhad city, province of khorasan ...

Domestic and sylvatic Trichinella genotypes were evaluated for infectivity, muscle larvae persistence, and host antibody responses in rats. Groups of rats were inoculated with T. spiralis, T. nativa, T. britovi, three genotypes of T. pseudospiralis (from USSR, USA, and Australia), T. murrelli, Trichinella T6, and T. nelsoni, respectively. The muscle larvae intensity (larvae per gram), total lar...

Hunting in Poland has a long tradition and became more popular after 1990. Each year over 60,000 wild boar are hunted. Some of them may act as Trichinella carriers thus all carcasses of wild boar are systematically sampled in game-handling establishments as part of the post-mortem examination. The aim of the study was to determine the species of Trichinella and to evaluate the year to year diff...