low phosphorous (p) availability in soils limits production of soybean [glycine max (l.) merr.] around the world. this study was conducted to determine whether exogenous expression of the rice (oryza sativa l.) phosphates transporter gene ospt2 would increase inorganic phosphates (pi) acquisition and improve yield in transgenic soybean. cotyledonary-node explants of the soybean were inoculated ...

Transformation of plants using Agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. In this study, T-DNA from Ri plasmid in A. rhizogenes carrying pRi15834-PRT35S-GUS was introduced into tobacco leaf segments to initiate development of transformed hairy roots. Plant regeneration from transgenic roots used MS medium, and plants regenerated fro...

Salinity is a major limiting factor for plant growth and development. To evaluate the impact of P5CS gene expression under in vitro salt stress condition, transgenic tobacco (Nicotiana plumbaginifolia) carrying P5CS gene and non-transgenic plants were treated with 0, 100, 150, 200 or 250 mM NaCl for 28 days. Proline content, lipid peroxidation and the activity of some antioxidant enzymes after ...

transformation of plants using agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. in this study, t-dna from ri plasmid in a. rhizogenes carrying pri15834-prt35s-gus was introduced into tobacco leaf segments to initiate development of transformed hairy roots. plant regeneration from transgenic roots used ms medium, and plants regenerated fro...

Cotton cultivar Coker has been already transformed with recombinant pBI121-chi via Agrobacterium tumefaciens. The T-DNA region of pBI121-chi carries the chitinase (chi ) gene from bean and is under the control of the CaMV35S promoter. T1 and T2 progenies of transgenic cotton containing the chi gene were used in this study.  Polymerase chain reaction (PCR), Southern and Western blotting data con...

pod borer is one of the main causes for yield loss of chickpea. therefore, breeding of chickpea for resistance to this pest is important. the use of cry toxin from bacillus thuringiensis is an effective strategy for producing of transgenic resistant chickpea to this pest. these toxins are able to become active in the midgut of larvae and disrupt the insect's digestive system. we studied the sta...

salinity is a major limiting factor for plant growth and development. to evaluate the impact of p5cs gene expression under in vitro salt stress condition, transgenic tobacco (nicotiana plumbaginifolia) carrying p5cs gene and non-transgenic plants were treated with 0, 100, 150, 200 or 250 mm nacl for 28 days. proline content, lipid peroxidation and the activity of some antioxidant enzymes after ...

the purpose of this study was to examine the effect of educational – extension factors on the use of transgenic plants. this study was conducted through a survey design. the research was an applied study type. the research method of this study is descriptive-correlation.  statistical population of the study consisted of all of biotechnology experts of research center of agriculture of ilam prov...