a stable agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [glycine max (l.) merr.]. the cotyledonary node explants of the soybean cultivar ‘ny-1001’ were inoculated with the agrobacterium tumefaciens strain eha105, harboring the vector pcambia3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...

it has been more than half a century that plant geneticists and breeders have been trying to assemble a combinationof genes in crop plants, in order to make them as suitable and productive as possible. plant transformation technology incrop plants was first undertakenin the 1980s based on the ability of foreign gene integration into host plant genome andregeneration of transformed plant cells i...

Introduction: PolG-alpha is a nuclear-encoded enzyme which provides replication and repair of mitochondrial DNA. D257A mutation leads to change in the N-terminal ”proofreading” domain, deprives 3?-5? exonuclease activity, resulting accumulation mutations genome.
 Materials methods: Murine zygotes were microinjected with transgene construction carrying mutant murine Polg coding sequence GFP...

Background: A highly efficient genetic transformation system is essential for a successful genetic manipulation of the African violet (Saintpaulia ionantha Wendl.). Objectives: Developing a particle bombardment-based genetic transformation system for the African violet. Materials and Methods: A local cultivar of the African violet from Guilan province was used for transformation experiments. Th...

The recent development of homologous recombination-mediated gene targeting (GT) techniques has made it easy to modify nucleotide sequences in plant genomes. Backcross breeding following GT provides transgene-free lines with high probability. However, owing the possibility unintentional transgene introduction during genetic transformation, analytical methods may be necessary reliably and compreh...

Sugarcane (Saccharum spp.) is one of the most valuable food and industrial crops. Its production constrained due to major biotic (fungi, bacteria, viruses insect pests) abiotic (drought, salt, cold/heat, water logging heavy metals) stresses. The ever-increasing demand for sugar biofuel rise new pest disease variants call use innovative technologies speed up sugarcane genetic improvement process...