given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. in this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. first, the contiguous sequencesare generated using de bruijn graph with d...

Given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. In this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. First, the contiguous sequencesare generated using de Bruijn graph with d...

one of the best strategies to control bacterial wilt caused by ralstonia solanacearum (smith) is generally based on breeding resistant cultivars. the information obtained from the expression of plant defense genes will provide new insight for improving plant resistance against pathogens. this study was to identify inducible genes under defense no death (dnd) reaction of tobacco (nicotiana tabac...

The transcriptome of triangle sail mussel Hyriopsis cumingii (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonad, hepatopancreas, foot, mantel, gill and adductor muscle, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48 % Q20 bases were generated. Cluster...

RNA-sequencing is a new method of transcriptome characterization of organisms. Based on identity and relatedness, there are large genetic variations among different cattle breeds. The goal of the current study was to sequence the transcriptome of Guilan native cow and compare with available reference genome using RNA-sequencing method. Blood samples were collected from 14 Guilan native cows and...

transition from vegetative to reproductive growth is an important stage in plant’s life. flowering pathways including photoperiod, vernalization, gibberellins, and autonomous pathway are regulated by different genes. identification of flowering genes is essential for the development of bolting-resistant sugar beet cultivars. in this study, a set of 118 arabidopsis thaliana genes involved in flo...

The transcriptome of triangle sail mussel Hyriopsis cumingii (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonads, hepatopancreas, foot, mantel, gills and adductor muscles, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48 % Q20 bases were generated. Clus...