transformation of plants using agrabacterium rhizogenes may affect secondary metabolite production as well as morphological changes. in this study, t-dna from ri plasmid in a. rhizogenes carrying pri15834-prt35s-gus was introduced into tobacco leaf segments to initiate development of transformed hairy roots. plant regeneration from transgenic roots used ms medium, and plants regenerated fro...

The present research is conducted to find out the services and facilities of tobacco companies in promotion of tobacco crop in Malakand division of Khyber Pakhtunkhwa, Pakistan. A multi-stage sampling technique applied for selection sample size. In this method, 135 tobacco growers were selected from fifteen villages of tobacco growers. The principle researcher interviewed selected respondents f...

By W. W. GARNER, Physiologist in Charge, J. E. MCMURTRëY, Assistant, C. W. BACON, Assistant Physiologist, Tobacco and Plant Nutrition Investigations, Bureau of Plant Industry, United States Department of Agriculture, and E. G. Moss, Assistant, Tobacco and Plant Nutrition Investigations, Bureau of Plant Industry, United States Department of Agriculture, and Assistant Director, Tobacco Branch Sta...

BACKGROUND: Nicotiana tobacco contains large amounts of alkaloid nicotine. Tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. Reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. Utilizing the new ...

By W. W. GARNER, Principal Physiologist in Charge^ J. E. MCMURTREY, JR., Associate Physiologist^ J. D. BOWLING, JR., Assistant Biochemist^ Office of Tobacco and Plant Nutrition, Bureau of Plant Industry, United States Department of Agriculture, and E. G. Moss, Senior Agronomist, Office of Tobacco and Plant Nutrition, Bureau of Plant Industry, United States Department of Agriculture, and Assista...

the experiment was done in a factorial design based on completely randomized with three factors and five replications in vitro. the first factors, plant extract (nine plant species), the second factor, solvent (5 solvent) and the third factor, concentration (3 concentration) were considered. in pot condition, the experiment was carried out in factorial based on completely randomized design with...

By W. W. GARNER, Principal Physiologist in Charge: J. E. MCMURTREY, Jr., Associate Physiologist; J. D. BOWLING, Assistant Biochemist; Office of Tobacco and Plant Nutrition, Bureau of Plant Industry, United States Department of Agriculture; and E. G. Moss, Senior Agronomist, Office of Tobacco and Plant Nutrition, Bureau of Plant Industry, United States Department of Agriculture, and Assistant Di...

background: nicotiana tobacco contains large amounts of alkaloid nicotine. tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. utilizing the new me...