this study was carried out to investigate the effects of dietary putrescine (put) on broiler’s response fed low crude protein (cp) diets. a total of 192 male day old chicks were fed with four dietary treatments including two levels of put (0 and 0.03%) and two levels of cp (normal and low) with factorial combinations. weekly growth performance, nutrient digestibility and intestinal morphology (...

in plants, γ-aminobutyric acid (gaba) is synthesized by polyamine degradation pathway besides gaba shunt. aminoguanidine (ag) is a specific inhibitor of the key enzyme (diamine oxidase, dao) for gaba formation in polyamine degradation pathway. in this study, ag was applied to study the functions of polyamine degradation pathway on growth and gaba accumulation in germinating fava bean under hypo...

alpha-Difluoromethylornithine (DFMO), an investigational chemopreventive agent, suppresses polyamine contents and decreases epithelial carcinogenesis in experimental models. The ability of this drug to decrease polyamine contents in human esophageal tissues has not yet been determined. Eight patients with Barrett's esophagus were treated with DFMO at a dose of 1.5 g/m2/day for 12 weeks. Four si...

Colorectal cancer (CRC) is one of the most frequent forms malignant tumors in human population. The literature data about role arginine metabolism CRC point out its double-faced role. In three tissue specimens 50 patients who underwent surgical resection for colon adenocarcinoma (tumor, adjacent and healthy tissues more than 10 cm from tumor border ? at incision margin) taken during surgery, po...

Senescence is an integral part of the normal developmental cycle of plants and can be traced in cell, tissue and organ level. This work was focused on the efficiency of post-harvest treatment with the free polyamine spermine (Spm) to extend the vase life of cut rose flowers as an anti-senescence compound. Spm treatment was applied at the rates of 0, 1, 2 and 4 mM. Some morphological, biochemica...

With the use of a newly developed and convenient enzymatic method, tissue, urine and blood polyamine (diamine, spermidine and spermine) levels were evaluated as a tumor marker of renal cell carcinoma (RCC) in 50 cases with the disease. Furthermore, blood and urine polyamines were periodically determined and evaluated as a follow-up marker. The pretreatment three polyamine levels in tissue, bloo...

In the organism, quiescent epithelial cells have the potential to resume cycling as a result of various stimuli, including wound healing or oxidative stress. Because quiescent cells have a low polyamine level, resuming their growth requires an increase of their intracellular polyamine levels via de novo polyamine synthesis or their uptake from plasma. Another alternative, explored here, is an i...

The polyamines spermidine and spermine, and their precursor putrescine, are required for cell growth and cellular functions. The high levels of tissue polyamines are implicated in carcinogenesis. The major sources of exogenous polyamines are diet and intestinal luminal bacteria in gastrointestinal (GI) tissues. Both endocytic and solute carrier-dependent mechanisms have been described for polya...

N,N'-Bis(benzyl)polyamine analogs were found to be substrates for highly purified polyamine oxidase. Metabolism of these analogs was apparently dependent on molecular O2 and resulted in the formation of benzaldehyde, H2O2, and a polyamine analog with free terminal amines. The debenzylation reaction was optimal between pH 9 and 10, identical to the pH optimum for polyamine oxidase activity when ...

Intracorporal injection of plasmids encoding opiorphins into retired breeder rats can result in animals developing a priapic-like condition. Microarray analysis demonstrated that following intracorporal gene transfer of plasmids expressing opiorphins the most significantly upregulated gene in corporal tissue was the ornithine decarboxylase gene (ODC). Quantitative RT-PCR confirmed the upregulat...