Cultured tissues and cells are used extensively in physiological and pharmacological studies. In vitro cultures provide a means of examining cells and tissues without the complex interactions that would be present if the whole organism were studied. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This unit describe...

In vitro culture of human ovarian tissue the following cryopreservation is proposed for follicular development. There are no techniques that guarantee successful maturation of the follicles within the excised tissue. The viability of cultured human ovarian tissue improved by adding some growth factors to the culture media. The efficiency of vitrification as the cryopreservation method for human...

plant tissue culture techniques were used to study the effect of nacl on tropane alkaloids accumulation and proline content of both datura metel and datura stramonium. callus cultures were established for both datura species. however, ms medium supplemented with 1mg/l of both ba and naa was the best for callus production in d. metel, while ms supplemented with 3 mg/l kin and 1 mg/l 2, 4-d was t...