background: the aim of this cross-sectional study was to investigate the relationship between body mass index (bmi), waist-to-hip ratio, and lipid parameters and serum thyroid-stimulating hormone (tsh) levels in healthy euthyroid individuals. methods: this cross-sectional study was conducted between june 1st and july 20th, 2013, at bushehr university of medical sciences. one hundred forty euthy...

Unlabeled cholera toxin inhibits [125I]thyrotropin binding to thyrotropin receptors on thyroid plasma membranes. Maximal inhibition by cholera toxin does not exceed 40%, whereas unalbeled thyrotropin completely inhibits [125I]thyrotropin binding to these same membranes. Kinetic analyses of the binding data are compatible with the view that the cholera toxin decreases the number of receptor site...

Previously reported experiments (Winand, R. J., and Kohn, L. D. (1970) J. Biol. Chem. 245, 967-975; Kohn, L. D., and Winand, R. J. (1971) J. Biol. Chem. 246, 6570-6575) have demonstrated that partial pepsin digestion of bovine thyrotropin preparation yields a fragment of the thyrotropin molecule which is exophthalmogenic but has negligible or no thyroid-stimulating activity. In the present repo...

OBJECTIVE To investigate the relationship between thyrotropin concentrations within the accepted reference range and cardiovascular risk. DESIGN Initially, 728 women aged 45-60 years were enrolled over a 12-month period. All participants underwent full cardiovascular assessment, including detailed health questionnaire, sphygomanometry, body mass index (BMI) calculation, fasting glucose and li...

A strongly fluorescent 5-dimethylamino-1-naphthalene sulfonate (dansyl) derivative of bovine thyrotropin has been prepared. The dye-conjugated hormone is bioactive and shares, essentially unchanged, the membrane binding and adenylate cyclase stimulatory activities of the native hormone. Binding of 125I-labeled dansyl-thyrotropin to thyroid plasma membranes is sensitive to inhibition by ganglios...

INTRODUCTION While thyrotropin-releasing hormone is known to be a prolactin-release stimulating factor, thyrotropin-releasing hormone-tartrate and its derivative, taltirelin hydrate, are used for the treatment of spinocerebellar degeneration, a degenerative disease characterized mainly by motor ataxia. We report the case of a patient being treated with a thyrotropin-releasing hormone preparatio...

receptors was investigated. IgG from Graves’ sera with high thyroid-stimulating antibody activity were diluted in IgG from a pool of normal human serum (so that each sample contained the same amount of total IgG) and incubated overnight at 0°C with labelled thyrotropin and membranes solubilized with 0.5 % Triton. The mixtures were then analysed by chromatography on Sepharose or by poly(ethy1ene...

OBJECTIVE To evaluate Michigan newborn screening for congenital hypothyroidism (CH) protocol changes. METHODS This population-based study includes infants born and screened in Michigan (January 1, 1994-June 30, 2010). Screening performance is compared across 4 periods defined by the dried blood spot testing method: (1) thyroxine (T4) with backup thyrotropin, (2) tandem T4 and thyrotropin, (3)...

Gangliosides inhibit 125I-labeled thyrotropin binding to the thyrotropin receptors on bovine thyroid plasma membranes, on guinea pig retro-orbital tissue plasma membranes, and on human adipocyte membranes. This inhibition by gangliosides is critically altered by the number and location of the sialic acid residues within the ganglioside structure, the efficacy of inhibition having the following ...