The stability of thermophilic proteins has been viewed from different perspectives and there is yet no unified principle to understand this stability. It would be valuable to reveal the most important interactions for designing thermostable proteins for such applications as industrial protein engineering. In this work, we have systematically analyzed the importance of various interactions by co...

INTRODUCTION Protein thermostability is an important field for its evolutionary perspective of mesophilic versus thermophilic relationship and for its industrial/ therapeutic applications. METHODS Presently, a total 400 (200 thermophilic and 200 mesophilic homologue) proteins were studied utilizing several software/databases to evaluate their amino acid preferences. Randomly selected 50 homol...

MOTIVATION Understanding the basis of protein stability in thermophilic organisms raises a general question: what structural properties of proteins are responsible for the higher thermostability of proteins from thermophilic organisms compared to proteins from mesophilic organisms? RESULTS A unique database of 373 structurally well-aligned protein pairs from thermophilic and mesophilic organi...

In this study, we used features that can be extracted from protein sequences to discriminate mesophilic, thermophilic and hyper-thermophilic proteins. Amino acid frequency, dipeptide amino acid frequency and physical-chemical features are used in this study. The effect of mentioned features on proposed discrimination algorithm was evaluated both separately and in combination. Statistical method...

In this work we employ various methods of analysis (unfolding simulations and comparative analysis of structures and sequences of proteomes of thermophilic organisms) to show that organisms can follow two major strategies of thermophilic adaptation: (i) General, non-specific, structure-based, when proteomes of certain thermophilic organisms show significant structural bias toward proteins of hi...

Many studies have been undertaken to characterise alcohol dehydrogenases (ADHs) from thermophiles and hyperthermophiles, mainly to better understand their activities and thermostability. To date, there are 20 thermophilic archaeal and 17 thermophilic bacterial strains known to have ADHs or similar enzymes, including the hypothetical proteins. Some of these thermophiles are found to have multipl...

INTRODUCTION .......... ....................................... 320 THE THERMOPHILIC MICROORGANISMS .......... ......................... 321 POSSIBLE MECHANISMS OF SURVIVAL .......... .......................... 322 Lipids ................................................. 322 Rapid Resynthesis ............ ..................................... 322 Thermally Stable Macromolecules ...................