tlc screening and evaluation of antioxidant, antibacterial activity of onopordon macrocephalum by bioautography methodonopordon known as scotch thistle is a medicinal plant of the asteraceae family that is widely distributed in europe and asia, also it has many valuable medicinal properties as hypotensive and antitumor. thin layer chromatography (tlc) is wildly used in natural product extract a...

TLC screening and Evaluation of antioxidant, antibacterial activity of Onopordon macrocephalum by bioautography methodOnopordon known as scotch thistle is a medicinal plant of the Asteraceae family that is widely distributed in Europe and Asia, also it has many valuable medicinal properties as hypotensive and antitumor. Thin layer chromatography (TLC) is wildly used in natural product ext...

Bioautography is a microbial detection method hyphenated with planar chromatography techniques. It is based mainly on antimicrobial or antifungal properties of analyzed substances. The review discusses three versions of bioautography, i.e. contact, immersion and direct bioautography. The more concern is given to the last one. Many applications are quoted, not only for testing various groups of ...

BACKGROUND Athrixia phylicoides DC. (Asteraceae) is used medicinally in South Africa to treat a plethora of ailments, including heart problems, diabetes, diarrhoea, sores and infected wounds. It is also prepared in the form of a tea (hot decoction) taken as a refreshing, pleasant-tasting beverage with commercialization potential. METHODS Extracts of the dried ground aerial parts were prepared...

S treptococcus mutans (Gram-positive) and higella dysenteriae (Gram-negative) are two types of pathogen bacteria. The use synthetic antibiotics against both bacteria is known to impact the bacteria's resistance. E. spinosum from Tasikmalaya a potential macroalgae as source an antibacterial compound for research aims determine metabolite s pinosum originated S. . was conducted through several st...

A common screen for plant antimicrobial compounds consists of separating plant extracts by paper or thin-layer chromatography (PC or TLC), exposing the chromatograms to microbial suspensions (e.g. fungi or bacteria in broth or agar), allowing time for the microbes to grow in a humid environment, and visualizing zones with no microbial growth. The effectiveness of this screening method, known as...

salvia genus is one of the largest genera of the lamiaceae family. its species have been used for a wide variety of disorders in the local traditional medicine systems. therefore, the genus has been the subject of several phytochemical and biological studies. the aim of the study was to identify the major antioxidant compound(s) from the methanol extract of salvia verticillata using activity-gu...

A new bioassay has been developed combining the simplicity of direct bioautography with the improved chromatographic resolution of 2D-TLC. Mixtures of structurally diverse antifungal agents were tested to establish the validity and utility of this method in the discovery of new natural products with activity against agriculturally important fungal pathogens.

PURPOSE Coleus amboinicus is a medicinal plant traditionally used to treat various diseases such as throat infection, cough and fever, diarrhea, nasal congestion and digestive problems. The plant was explored for endophytic fungi producing antimicrobial agents. METHODS Screening for endophytic fungi producing antimicrobial agents was conducted using agar plug method and antimicrobial activity...

Interest in the antioxidant activity of bamboo leaves is growing. To discover new sources of natural antioxidants, a TLC bioautography method combined with a new image processing method was developed to evaluate the antioxidant activity of leaf extracts from 15 different species of bamboo. The results showed that the methanolic extract of Bambusa. textilis McClure possessed the highest antioxid...