Meyveler arasında muz (Musa sp.) Dünyadaki tropikal ve subtropikal tarımın temel dayanağıdır. Muzun meyvesi yenir kabuğu atılır. Ancak, kabuğunun sağlığa olan faydaları, son birkaç on yılda geleneksel tıptaki birçok hastalığın tedavisinde çok dikkat çekti. Literatür, antifungal, antioksidan, antibiyotik antidiyabetik gibi farmakolojik aktivitelerini bildirmiştir. Bu çalışmada, Brezilya (Bbp) Tü...

Introduction TATA box-binding protein (TBP) plays a central role in gene expression through the interactions with RNA polymerase and gene-specific transcription factors. As TBP is one of the common components of eukaryotic and archaeal transcription initiation, the investigation of the structural and functional relationship of TBP in more detail is the most attractive strategy to understand the...

The interaction of the general transcription factor (TF) IIA with TFIID is required for transcription activation in vitro. TFIID consists of the TATA-binding protein (TBP) and TBP associated factors (TAFIIs). TFIIA binds directly to TBP and stabilizes its interaction with TATA-containing DNA. In this work, we present evidence that TAFIIs inhibit TBP-DNA and TBP-TFIIA binding, and that TFIIA sti...

The TATA-binding protein TBP has been recently recognized as a general class III transcription factor. Using the gel shift assay to monitor initiation complex assembly on a yeast tRNA gene, we show that TBP is required for the TFIIIC-dependent assembly of TFIIIB. TFIIIB depleted of TBP by a simple chromatographic step does not bind stably to the TFIIIC-tDNA complex. Addition of yeast or human r...

The TATA binding protein (TBP) is an essential transcription initiation factor in Archaea and Eucarya. Bacteria lack TBP, and instead use sigma factors for transcription initiation. TBP has a symmetric structure comprising two repeated TBP domains. Using sequence, structural and phylogenetic analyses, we examine the distribution and evolutionary history of the TBP domain, a member of the helix-...

Essentially all nuclear eukaryotic gene transcription depends upon the function of the transcription factor TATA-binding protein (TBP). Here we show that the abundant, multifunctional DNA binding transcription factor repressor activator protein Rap1p interacts directly with TBP. TBP-Rap1p binding occurs efficiently in vivo at physiological expression levels, and in vitro analyses confirm that t...

In mammalian and Drosophila cells, the central RNA polymerase II general transcription factor TFIID is a multisubunit complex containing the TATA-binding protein (TBP) and TBP-associated factors (TAFs) bound to the conserved TBP carboxy-terminal core domain. TBP also associates with alternative TAFs in these cells to form general transcription factors required for initiation by RNA polymerases ...

In yeast, the multisubunit SAGA (Spt-Ada-Gcn5-acetyltransferase) complex acts as a coactivator to recruit the TATA-binding protein (TBP) to the TATA box, a critical step in eukaryotic gene regulation. However, it is unclear which SAGA subunits are responsible for SAGA's direct interactions with TBP and precisely how SAGA recruits TBP to the promoter. We have used chemical crosslinking to identi...

The synergistic solvent extraction system comprising tri-n-butyl phosphate (TBP) and FeCl3 has been intensively studied for selective of Li(I) from Mg(II)-rich brine. occurs via the formation an ion-pair complex [Li(TBP)x][FeCl4] in which negatively charged [FeCl4]? neutralizes positively [Li(TBP)x]+. Counterintuitively, many other metal chlorides give much lower efficiency than FeCl3, although...

The recruitment of TATA binding protein (TBP) to gene promoters is a critical rate-limiting step in transcriptional regulation for all three eukaryotic RNA polymerases. However, little is known regarding the dynamics of TBP in live mammalian cells. In this report, we examined the distribution and dynamic behavior of green fluorescence protein (GFP)-tagged TBP in live HeLa cells using fluorescen...