the ph dependence study reveals that the cys 206 sulphydryl group of bovine carbonicanhydrase in the native form is not exposed. during the reaction of 2,2'-dithiobispyridine (2-dtp) with the enzyme, there was no absorbance change recorded. in the presence ofsurfactants, the ph dependence profiles of the apparent second order rate constants, kapp, forthe reaction of 2-dtp with bovine carbonic a...

the ph dependence study reveals that the cys 206 sulphydryl group of bovine carbonicanhydrase in the native form is not exposed. during the reaction of 2,2'-dithiobispyridine (2-dtp) with the enzyme, there was no absorbance change recorded. in the presence ofsurfactants, the ph dependence profiles of the apparent second order rate constants, kapp, forthe reaction of 2-dtp with bovine carbo...

Dog hemoglobin has four sulphydryl groups at positions olfl (G18) and p93 (F9), all of which are titratable with mercurials. Only two of these, however, react with non-mercurial sulphydryl reagents. Kinetic results indicate that the reacting site might be the p”” (F9). An examination of the environment of the alI1 (G18) shows that this sulphydryl must be unreactive towards non-mercurials becaus...

The pH dependence study reveals that the Cys 206 sulphydryl group of bovine carbonicanhydrase in the native form is not exposed. During the reaction of 2,2'-dithiobispyridine (2-DTP) with the enzyme, there was no absorbance change recorded. In the presence ofsurfactants, the pH dependence profiles of the apparent second order rate constants, kapp, forthe reaction of 2-DTP with bovine carbonic a...

The reactivities of 5,5’-Dithiobis (2-nitrobenzoate) with the sulphydryl groups of major haemoglobin of duck (Anas platyrhinchos) have been carried out in the presence of inositol hexakisphosphate, inositol-P6. The time course of the reaction is biphasic. The addition of inositol-P6 changed the pH dependence profile of the apparent second-order rate constant of both oxyand carbonmonoxyhaemoglob...

The changes in plasma protein sulphydryl level were measured during the course of adjuvant-induced arthritis in rats. Major depressions in the plasma sulphydryl level occurred at the onset of adjuvant disease, and the extent of the depression was related to the severity of the disease. Plasma sulphydryl levels remain unchanged when the systemic arthritis is suppressed by inclusion of a competin...

Parachloromercury benzoate (PCMB), a sulphydryl inactivator, caused a progressively increasing inhibition of tissue responses to acetylcholine, adrenaline, noradrenaline and serotonin in vitro. This inhibition was progressively and completely reversed by penicillamine, a sulphydryl activator. It is inferred that intact sulphydryl groups are essential for constancy of responses of excitable tiss...

The prolonged administration of the sulphydryl compound penicillamine to rheumatoid arthritis patients results in a fall in the titre of rheumatoid factor (Jaffe, 1963). The mechanism by which the drug produces this effect is unknown, though it does not appear to be by intravascular depolymerization of macroglobulins (Jaffe, 1964) or by immunosuppression by pyridoxine antagonism (Jaffe, 1965). ...