conclusions single dose therapy of mtx has sufficient power and efficacy in the treatment of ectopic pregnancy, however in patients with higher serum level of β-hcg, the successful treatment increases by using double dose method. using double dose also could decrease the necessity of operation, re-administration of mtx, and duration of hospitalization. results results showed that the rate of su...

background: rotaviruses (rv) are important viral diarrheal agents in calves. vaccination is an optimum measure to prevent bovine rotaviruses (brv) infection. however, little research on brv vp7 vaccine has been done and currently there is no brv vaccine. objective: to prepare a subunit vaccine of brv and investigate its efficacy. methods: total rna was extracted from ma104 cells infected with b...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

background: no consensus has been reached on prognostic value of serum concentration of β (beta) subunit of human chorionic gonadotropin (β-hcg) in treatment response to methotrexate in management of ectopic pregnancy. therefore, the present study aimed to evaluate this subject through a systematic review and meta-analysis. materials and methods: an extensive literature search on online databas...

brucellosis is a well-known infectious disease among domestic animals caused by brucella bacterium. omp25 is an outer membrane protein of brucella which plays an important role in immunogenicity of cells. moreover, brucella lumazine synthase (bls) as another main antigen can be used as an adjuvant when covalently attached to a foreign antigen. in the current study, designing and production of a...

background: sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. ectodomain of influenza m2 protein is a surface and highly conserved protein. m2e in influenza vaccines may eliminate the need for changing vaccine formulation every year. objectives: in this study, a recombinant baculovirus containing m2e and cholera toxin subunit b fus...

Tetrodotoxin (TTX)-binding proteins are present in toxic TTX-bearing animals, such as pufferfish and gastropods. These may prevent autotoxicity. However, TTX-binding also found the nontoxic marine shore crab, Hemigrapsus sanguineus. Here, we isolated protein, HSTBP (Hemigrapsus sanguineus protein), from hemolymph of H. elucidated its primary structure using cDNA cloning. HSTBP, a 400 kDa acidic...

cholera toxin b subunit (ctxb) is a homopantameric, nontoxic subunit of cholera toxin that is responsible for its binding to the cell and has been known as a mucosal adjuvant for vaccines that could increase homoral and mocusal immunity response. in this work, the ctxb gene was fused to the stxb gene from shigella dysenteriae type i a vaccine antigen candidate against this pathogen, by a nonfur...