We describe here the draft genome sequence of Sporosarcina pasteurii, a urease-producing bacterium with potential applications in biocement production.

In this study, we have introduced a biological dust control technique utilizing a naturally occurring soil microorganism, Sporosarcina pasteurii, which is capable of inducing calcium carbonate precipitation in the environment. To evaluate the dust suppressive potential of this microbial calcite, S. pasteurii was suspended in medium and applied to locally available sand. The treated soil samples...

In this study, five microbes were employed to precipitate calcite in cohesionless soils. Four microbes were selected from calcite-precipitating microbes isolated from calcareous sand and limestone cave soils, with Sporosarcina pasteurii ATCC 11859 (standard strain) used as a control. Urease activities of the four microbes were higher than that of S. pasteurii. The microbes and urea-CaCl₂ medium...

AIMS Ureolysis drives microbially induced calcium carbonate precipitation (MICP). MICP models typically employ simplified urea hydrolysis kinetics that do not account for cell density, pH effect or product inhibition. Here, ureolysis rate studies with whole cells of Sporosarcina pasteurii aimed to determine the relationship between ureolysis rate and concentrations of (i) urea, (ii) cells, (iii...

Urease activation is critical to the virulence of many human and animal pathogens. Urease possesses multiple, nickel-containing active sites, and UreE, the only nickel-binding protein among the urease accessory proteins, activates urease by transporting nickel ions. We performed NMR experiments to investigate the solution structure and the nickel-binding properties of Bacillus pasteurii (Bp) Ur...

Using a semi-solid 0.5% agar column, we study the phenomenon of microbially induced mineral (calcium carbonate) by the bacteria Sporosarcina pasteurii. Our platform allows for in-situ visualization of the phenomena, and we found clear evidence of bacterial cell surface facilitating formation of nanoscale crystals. Moreover, in the bulk agar we found the presence of microspheres, which seem to a...

Biological processes may provide great and previously unexplored opportunities for cost-effective, in situ improvement of the engineering properties of soil. A laboratory study was conducted to evaluate the changes in geomechanical properties of sand attributable to the formation of calcium precipitates induced through ureolysis catalyzed by Sporosarcina pasteurii (S. pasteurii). Specifically, ...

Bioremediation using microorganisms is a promising technique to remediate soil contaminated with heavy metals. In this study, Sporosarcina pasteurii (S. pasteurii) bioremediation by mixing method was used soils lead (Pb), zinc (Zn) and cadmium (Cd). A significant reduction of metal leaching concentrations observed in S. bio-treated samples. Furthermore, urease hydrolyzing bacteria have addition...