an on-line uv-hplc / apci-ms (atmospheric pressure chemical ionization) tandem by a platinium c18 column with an isocratic solvent system after optimizing was applied to the separation and the identification of different carotenoids including astaxanthin, canthaxanthin, apocarotenoic ester, torularhodin and beta carotene. this method was used to identify particular carotenoids produced by newly...

a new isolated strain of sporobolomyces ruberrimus h110 was cultivated  on technical glycerol as carbon source and effect of different temperatures and ph on the growth and pigmentation was studied. the maximum concentration of total carotenoid was 3.84 mgg-1 including torularhodin (3.70 mgg-1) and β-carotene (0.14 mgg-1) using 19ºc at ph 6, but the highest amount of the maximum specific growth...

An on-line UV-HPLC / APCI-MS (atmospheric pressure chemical ionization) tandem by a platinium C18 column with an isocratic solvent system after optimizing was applied to the separation and the identification of different carotenoids including astaxanthin, canthaxanthin, apocarotenoic ester, torularhodin and beta carotene. This method was used to identify particular carot...

abstract in this study, the extraction of carotenoids from a new strain of yeast (sporobolomyces ruberrimus h110) by different chemical and physical methods was investigated. for chemical pretreatment methods, hcl at two different concentrations (0.2 and 0.5 n) and two different temperatures (55 and 75c), and dmso solvent (also at two different temperatures, 55 and 75c) were used. sonication an...

A new isolated strain of Sporobolomyces ruberrimus H110 was cultivated  on technical glycerol as carbon source and effect of different temperatures and pH on the growth and pigmentation was studied. The maximum concentration of total carotenoid was 3.84 mgg-1 including torularhodin (3.70 mgg-1) and β-carotene (0.14 mgg-1) using 19ºC at pH 6, but the highest ...

Carotenoids are intracellular pigments produced by microorganisms, including some species of yeasts, in the stationary phase of growth by the secondary metabolic pathways. In the present study, different methods of Sporobolomyces ruberrimus H110 yeast cell lysis were evaluated with the objective of optimizing the recovery of intracellular pigments. Three extraction methods were used: vortex (gl...

In Ralstonia eutropha H16, the nitric oxide (NO)-responsive transcriptional activator NorR controls the expression of a dicistronic operon that encodes a membrane-bound NO reductase, NorB, and a protein of unknown function, NorA. The N-terminal domain (NTD) of NorR is responsible for perception of the signal molecule, nitric oxide. Thirteen out of 29 conserved residues of the NTD were exchanged...

Objective: Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder that causes progressive loss of the upper and lower neurons in brain spinal cord. SOD1 was first gene linked to ALS, which more than 150 mutations throughout sequence protein have been found be associated with ALS. Methods: The drugs can interact inhibit misfolding or revert misconformation useful treatment Monomer ...

I propose a quantitative methodology to analyze the economic impact of e-government based on structural modeling, allowing for a careful description of the underlying theoretical assumptions and for an assessment of different policy scenarios. The transparent relation between the theory and the results obtained is an advantage with respect to purely narrative methods. The methodology departs si...

Drs Nestor and Cook are from University of Kentucky HealthCare, Pharmacy Services, and Drs Ryan and Cook are from University of Kentucky College of Pharmacy, Lexington, Kentucky. Drs Nestor, Ryan, and Cook have no relevant fi nancial relationships to disclose. Correspondence should be addressed to: Aaron M. Cook, PharmD, BCPS, University of Kentucky HealthCare, 800 Rose St, H110, Lexington, KY ...