Intelligent knowledge based system (IKBS) is developed for optimizing dry CNC turning process using Taguchi method, CNC Machine, EN19 steel as the work piece material, andCutting Insert. Tool wear and spindle loading which are the machining parameters, spindle speed, feed rate, and depth of cut, areoptimized through the intelligent knowledge based system (IKBS). The experimental CNC turning mac...

Intelligent knowledge based system (IKBS) is developed for optimizing dry CNC turning process using Taguchi method, CNC Machine, EN19 steel as the work piece material, andCutting Insert. Tool wear and spindle loading which are the machining parameters, spindle speed, feed rate, and depth of cut, areoptimized through the intelligent knowledge based system (IKBS). The experimental CNC turning mac...

Equal chromosome segregation requires proper assembly of many proteins, including Bub3, onto kinetochores to promote kinetochore-microtubule interactions. By screening for mitotic regulators in the spindle envelope and matrix (Spemix), we identify a conserved Bub3 interacting and GLE-2-binding sequence (GLEBS) containing ZNF207 (BuGZ) that associates with spindle microtubules and regulates chro...

Spindle alignment is the process in which the two spindle poles are directed toward preselected and opposite cell ends. In budding yeast, the APC-related molecule Kar9 is required for proper alignment of the spindle with the mother-bud axis. We find that Kar9 localizes to the prospective daughter cell spindle pole. Kar9 is transferred from the pole to cytoplasmic microtubules, which are then gu...

In early mitosis, microtubules can be generated at kinetochores as well as at spindle poles. However, the role and regulation of kinetochore-derived microtubules have been unclear. In general, metaphase spindle microtubules are oriented such that their plus ends bind to kinetochores. However, we now have evidence that, during early mitosis in budding yeast, microtubules are generated at kinetoc...

The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB l...