background: ubiquitin, an 8.5 kda peptide that marks other proteins for proteasomal degradation, tags defective sperm dur­ing epididymal passage. thus, sperm ubiquitination is a universal marker for sperm defects and can be used as a sperm func­tion test. the objective of the present study was to examine the relationships between sperm ubiquiti­nation and clinical se­men parameters, using simpl...

conclusions sperm quality extracted by percutaneous pesa and tese procedures increases fertility outcomes compared to naturally ejaculated sperm in men with idiopathic co. more specifically, embryo quality, which is most relevant to fertility outcome, improved when surgically extracted sperm was used for icsi. results there was a significant difference in fertilization rate, embryo quality, imp...

background: pentoxifylline (px) prevents camp breakdown by inhibiting the activity of the camp-phosphatase and presumably, stimulates sperm motion. incubation with px causes hyperactivation of sperm, an important step in achieving fertilization, and leads to changes in membranes associated with sperm capacitation. objective: the purpose of this study was to examine the effects of pentoxifylline...

Here we describe an improved method of separating sperm cells from lysed epithelial cells via acoustic cell trapping. In particular, we show that in the processing of samples with low numbers of sperm cells, addition of polymeric beads to the sample[1] allowed for better performance in terms of higher trapping efficiency versus our previous acoustic differential extraction (ADE) results[2]. We ...

Different protocols incorporating methyl-β-cyclodextrin (MBCD) and reduced glutathione (GSH) have been reported to improve IVF recovery of cryopreserved mouse sperm on a C57BL/6 (J and N) genetic background. However, it is not clear which IVF protocol is most appropriate when using the various methods to cryorecover sperm with different sperm quality and sample volumes. Therefore, in the presen...

BACKGROUND The majority of men find the production of a semen sample an embarrassing and stressful experience. Consequently, the availability of an over-the-counter home sperm test, which would reliably and accurately allow the patient to obtain an assessment of fertility potential at their convenience, would be a major benefit. Our objective was to develop and evaluate a home sperm test that p...

Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes preferential lysis of epithelial contaminants followed by collection of intact sperm by centrifugation. While effective for samples where sperm ar...

the objective of the research was to test the effect of iron and aluminium coagulants commonly used for lake re-cultivation, on the pike sperm motility and fertilisation. the coagulants caused changes in the analyzed hydrochemical parameters of the water such as: fe, mg2+, tss, so42- and cl-. selected parameters of sperm motility: mot, vcl, vsl, vap, lin were estimated using casa. the sperm mot...

BACKGROUND Cryptozoospermia (CO) is a situation in which spermatozoa cannot be observed in a fresh semen sample unless an extended centrifugation and microscopic search are performed. CO patients are suggested to use only intracytoplasmic sperm injection (ICSI) as infertility treatment. But still there is debate about the choice of sperm source in cryptozoospermic men candidate for ICSI. OBJE...