sperm selection techniques have became an important tool to improve sub fertile ejaculates. the main aim of this study was to assess the dynamics of motile sperm subpopulations of ejaculates from subfertile stallions subjected to density gradient centrifugation (dgc) involving a double layer colloid [dgc2], before and after storage for 24 and 48 hours. ejaculates from eight stallions with ferti...

Background: Selection of human spermatozoa prior to assisted reproduction is currently based on motility and morphology. Recently, Zeta method has been introduced for normal sperm selection based on sperm surface charges. In addition, one of the features of apoptosis is the externalization of phosphatidylserine (PS), which are normally present on the inner leaflet of the sperm plasma membrane. ...

Background: Intracytoplasmic sperm injection (ICSI) can be considered the solstice in vitro insemination technique because it has powerfully allowed the treatment of male factor infertility, but in this procedure, except visual morphological selection, there is no standardization for sperm selection. Recently, hypo osmotic swelling test (HOST) has been proposed to have the potential to select i...

although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. the methods of sperm separation that have been repeatedly reported to isolate fractions rich in x- or y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. however, the ability of the se...

objective: the aim of this study was to examine the efficiency of ha-binding test and sperm gradient processing method for the selection of sperm with normal protamine, intact dna and normal morphology. materials and methods: the study was conducted on 29 patients undergoing icsi. semen analysis was carried out according to who criteria. semen samples were divided into three equal portions. one...

background: sperm selection for icsi based on morphology and motility might not be relevant to chromatin integrity. thus sperm selection based on sperm characteristics has been suggested. objective: the aim of this study was to compare the efficiency of zeta method with routine density gradient centrifugation method (dgc) for the selection of sperm with higher dna integrity. materials and metho...

Background: During spermatogenesis the sperm nucleus exchange histones against cystein rich protamines, which stabilize the sperm chromatin and protects the DNA against damages. At ejaculation the age of the patient, handling routines, processing of the semen sample and the storage of the final sperm suspension might damage the DNA. Sperm chromatin stability and DNA damages are evaluated with d...

Background: During ICSI, motile sperm with normal morphology are selected and injected into oocyte. But integrity of chromatin is not observable. Therefore, researchers are trying for to present novel method of sperm selection with high percentage of normal chromatin. HOST test is a useful assay of functional integrity of the human sperm membrane. We decide to use HOST test after DGC for infert...

sperm chromatin integrity has been being recognized as an important factor in male fertility. during normal fertilization, high quality sperm with intact chromatin are selected through natural selection in journey from vagina to fallopian tube. however, using assisted reproductive techniques, particularly icsi, the natural selection is bypassed. therefore sperm with dna breakage have the opport...