introduction: varicocele is considered as a common cause of male infertility. the aim of this study was to evaluate the effects of varicocelectomy on sperm parameters, sperm membrane integrity and sperm nuclear maturity. materials and methods: in this study along with routine semen analysis, hypo-osmotic swelling test (host), and aniline blue staining for assessment of as 45 and 90 days after i...

Background: Plant growth regulators gave entry into animals and human cells through diet. Gibberellic acid (GA3) is an extensively prevalent plant growth regulator due to its use in agriculture. Therefore current study was designed to investigate the effect of GA3 compound on sperm DNA integrity, nuclear maturity and motility. Materials and Methods: To follow-up current study 12 mature male s r...

introduction: standard semen analysis is routinly used to evaluate the male fertility, however some researchers belive that sperm functional test should also be used with standard semen analysis for more precise evaluation of male fertility. the aim of this study was to consider the relation between different nuclear maturity tests and in vitro fertilization, in order to select the nuclear matu...

Background: Varicocele has been repeatedly implicated as a cause of infertility in selected men. Previous studies have showed high levels of seminal oxidative stress, as evidenced by increased levels of ROS and reduced total antioxidant capacity in varicocele men, suggesting that sperm dysfunction in varicocele patients may be partly related to oxidative stress. Thus current study was designed ...

introduction: semen parameters and specially sperm morphology is used for the assessment of semen sample and for the prediction of the fertilization. however there is semen samples with normal sperm morphology but with hidden sperm chromatin anomalies. since sperm morphology and chromatin anomalies reduce the fertilization rat independently, the purpose of this study is to consider the relation...

objective: melatonin, the chief secretory product of the pineal gland, regulates dynamic physiological adaptations that occur in seasonally breeding mammals as a response to changes in daylight hours. because of the presence of melatonin in semen and the membrane melatonin receptor in spermatozoa, the impact of melatonin on the regulation of male infertility is still questionable. the aim of th...

Individual spermatozoa were assessed with pairs of probes for persistent histones and cytoplasmic retention, persistent histones and DNA fragmentation, and persistent histones and apoptotic markers. The individual spermatozoa were treated sequentially with combinations of probes for these cytoplasmic and nuclear biochemical markers. Sperm fields were recorded with computer-assisted imaging, and...

OBJECTIVE Melatonin, the chief secretory product of the pineal gland, regulates dynamic physiological adaptations that occur in seasonally breeding mammals as a response to changes in daylight hours. Because of the presence of melatonin in semen and the mem- brane melatonin receptor in spermatozoa, the impact of melatonin on the regulation of male infertility is still questionable. The aim of t...

Interspecific androgenetic rosy barb (Puntius conchonius) was generated using its cadaveric (-20 degrees C) or fresh sperm to activate nuclear genome inactivated oocytes of gray tiger barb (Puntius tetrazona). UV irradiation was used to inactivate nuclear genome of tiger barb oocytes. Thermal shock restored diploidy of rosy barb in the oocytes of tiger barb. Survival of androgenotes was 14% or ...

The various sperm cell types present in a simple ejaculate differ in their motility and morphology. However, little is known about the nuclear maturity of these sperm cells and their relationship with morphological and motile characteristics. Protamines are considered a good marker of sperm nuclear maturity since they are added to the DNA in the last stage of spermatogenesis. We have analyzed t...