Background Although the depth of the counting chamber is an important factor influencing sperm counting, no research has yet been reported on the measurement and comparison of the depth of the chamber. We measured the exact depths of six kinds of sperm counting chambers and evaluated their accuracy. MaterialsAndMethods In this prospective study, the depths of six kinds of sperm counting chamber...

background: although the depth of the counting chamber is an important factor influencing sperm counting, no research has yet been reported on the measurement and comparison of the depth of the chamber. we measured the exact depths of six kinds of sperm counting chambers and evaluated their accuracy. materials and methods: in this prospective study, the depths of six kinds of sperm counting cha...

BACKGROUND Although the depth of the counting chamber is an important factor influencing sperm counting, no research has yet been reported on the measurement and comparison of the depth of the chamber. We measured the exact depths of six kinds of sperm counting chambers and evaluated their accuracy. MATERIALS AND METHODS In this prospective study, the depths of six kinds of sperm counting cha...

With the development of the Makler counting chamber, the reproducibility and accuracy of computer-assisted semen analysis and of manual determination of sperm concentration and sperm motility were significantly enhanced beyond what is possible with other methods, such as the hemocytometer, the Horwell chamber, the Petroff-Hausser chamber, and Chartpak slides. The use of Micro-Cell, a disposable...

AND CONCLUSION In this study various quality aspects of the CellVision, Leja, and MicroCell disposable sperm cell counting slides were investigated. The height of the chambers was determined using interferometric spectrometry. The average heights of the Leja and MicroCell were very close, respectively 20.0 μm and 20.3 μm, whereas the CellVision was lower than specification (17.9 μm). The accura...

Objective: To compare two suites of culture media (one with HSA and one protein-free (PF) supplemented with methylcellulose) for washing human sperm in IVF. Methods: Semen samples (n = 41) underwent parallel density gradient preparation using PF or HSA-supplemented culture medium and subsequent yield, survival, morphology and motility were compared. Results: The PF medium resulted in a signific...

BACKGROUND Automated systems have enabled the counting of particles in urine to be standardized. Their superiority over traditional sediment analysis has been well documented, but they have not gained wide acceptance. The reasons for this are that sediment analysis has been performed and interpreted for decades. Additionally, pathologic casts and other unknown particles still must be confirmed ...

Sperm counts have been linked to several fertility outcomes making them an essential parameter of semen analysis. It has become increasingly recognised that Computer-Assisted Semen Analysis (CASA) provides improved precision over manual methods but that systems are seldom validated robustly for use. The objective of this study was to gather the evidence to validate or reject the Sperm Class Ana...

Sperm counts have been linked to several fertility outcomes making it an essential parameter of semen analysis. It has become increasingly recognised that Computer Assisted Semen Analysis provides improved precision over manual methods but systems are seldom robustly validated for use. The objective of this work was to gather the evidence to validate or reject the Sperm Class Analyser as a tool...