abstract background: improvements in culture media formulations have led to an increase in the ability of sheep embryo in culture throughout the preimplantation period. objective: this study was carried out to evaluate the effects of various concentrations of mem vitamins during in vitro maturation of sheep oocytes and subsequent embryo development. materials and methods: sheep ovaries were col...

Due to economical and scientific limitations, sheep embryo reproductive technologies are less commercially applied than in other animal species. However, it is very clear that, in the near future, those techniques are expected to have a central role in animal production as a consequence of genetic and reproductive demands. One drawback is that results obtained after sheep embryo cryopreservatio...

Background: This study was carried out to evaluate the pattern of reactive oxygen species (ROS) variations during in vitro oocyte maturation and embryo development in sheep, and to investigate whether embryo culture condition and the presence of cell-permeable superoxide dismutase mimetic [Manganese (III) meso-tetrakis (4-benzoic acid) porphyrin (MnTBAP)] can influence ROS pattern. Materials an...

This study investigated whether the transmission of naturally occurring scrapie in sheep can be prevented using embryo transfer. Embryos were collected from 38 donor ewes in a Suffolk sheep flock with a high incidence of naturally occurring scrapie, treated with a sanitary procedure (embryo washing) recommended by the International Embryo Transfer Society and then transferred to 58 scrapie-free...

The purpose of this investigation was to study and compare the electrophoretic patterns of optic lensproteins of different species of domestic animals at pre- and post-natal ages. Optic lenses were removed from the embryo or adult sheep, cattle, goat, camel and chicken at the slaughter-house then homogenized and subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). I...

background to current knowledge, different oocyte's recovery method and various seasons have profound impact on in vitro embryo production (ivep). objectives the aim of this study was to define an efficient recovery method for oocytes harvesting from slaughterhouse material in different seasons, and their effects on ivep yield. materials and methods ovaries from slaughtered ewes in breeding sea...

This review focuses on the platform of recent advances in reproductive biotechnologies in sheep and goat. Here, we discuss some aspects of veterinary and biomedical importance in artificial insemination using sexed semen, in vitro/in vivo fertilization, interspecies in vitro fertilization, multiple ovulation and embryo transfer (MOET), somatic cell nuclear transfer and transgenic animal product...

This paper reviews the status of embryo transfer and the major technologies applied to preimplantation of embryos in sheep. Embryo production from superovulated ewes is hindered by an unpredictable response to hormonal treatment. Progress in this area should be expected by an appropriated control of follicular development with gonadotropin-releasing hormone (GnRH) agonist or antagonist prior to...

the purpose of this investigation was to study and compare the electrophoretic patterns of optic lensproteins of different species of domestic animals at pre- and post-natal ages. optic lenses were removed from the embryo or adult sheep, cattle, goat, camel and chicken at the slaughter-house then homogenized and subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (sds-page). i...

This review summarizes the latest advances and main limitations for the implementation of in vitro embryo production programs in sheep and goats. We describe the laparoscopic assisted technique for oocyte retrieval and propose new insights for follicular manipulation to improve oocyte quality. Further description of the routine conducted in our laboratory for the in vitro process of oocyte matu...