abstract background: increase in nitric oxide (no) concentration accompanied by alteration in peritoneal immune defense reactions is involved in the pathogenesis of endometriosis. asymmetric dimethylarginine is an endogenous competitive inhibitor of no synthase. this study was designed to compare no metabolite (nitrite), asymmetric dimethylarginine, and estradiol concentrations in serum and per...

A new hydroxylated metabolite of tamoxifen, Metabolite Y [trans-1-(p-beta-hydroxyethoxyphenyl)-1,2-diphenylbut-1-ene] was characterized and subsequently measured by high-performance liquid chromatography in serum from patients receiving normal (10 mg twice daily) and high dose (greater than or equal to 150 mg twice daily) tamoxifen therapy for treatment of advanced breast cancer. In normal-dose...

A new hydroxylated metabolite of tamoxifen, Metabolite Y [frans-1 -(p-/?-hydroxyethoxyphenyl)-1,2-diphenylbut-1-ene] was characterized and subsequently measured by high-per formance liquid chromatography in serum from patients receiv ing normal (10 mg twice daily) and high dose (>150 mg twice daily) tamoxifen therapy for treatment of advanced breast cancer. In normal-dose patients, the serum le...

Abstract Background Both serotonergic signalling disruption and systemic inflammation have been associated with the pathogenesis of Alzheimer’s disease (AD). The common denominator linking two is catabolism essential amino acid, tryptophan. Metabolism via tryptophan hydroxylase results in serotonin synthesis, whilst metabolism indoleamine 2,3-dioxygenase (IDO) kynurenine its downstream derivati...

Several metabolites of tamoxifen, including 4-hydroxy-N-desmethyltamoxifen (metabolite BX), 4-hydroxytamoxifen (metabolite B), N-desmethyltamoxifen (metabolite X), the primary alcohol (metabolite Y), and N-desdimethyltamoxifen (metabolite Z) were identified and their concentrations determined in fluids and feces from patients receiving chronic tamoxifen treatment. The biological samples investi...

We evaluated four commercially available 125I-digoxin radioimmunoassay kits with regard to their ability to cross react with the digoxin metabolite dehydrodigoxin. We prepared dihydrodigoxin serum samples in digoxin-free serum over the concentration range 0.4 to 5.0 microgram/liter and assayed them with each kit according to the manufacturer's instructions. The metabolite was able to displace t...

OBJECTIVE The human serum metabolite profile is reflective of metabolic processes, including pathophysiological changes characteristic of diseases. Therefore, investigation of serum metabolite concentrations in obese children might give new insights into biological mechanisms associated with childhood obesity. METHODS Serum samples of 80 obese and 40 normal-weight children between 6 and 15 ye...