Background Recent studies indicate increased serum amylase in children with acute appendicitis. The objective of the current study was to determine the diagnostic accuracy of raised serum amylase level in prediction of perforated appendicitis in children with acute appendicitis. Materials and Methods In this cross-sectional study, 61 children (aged 3 to 15 years) who underwent appendectomy su...

Pure amylase was isolated from pancreata and parotid glands of the baboon, an animal which has a serum amylase level and renal clearance of amylase (C(Am)) similar to man. After bolus injection, both pancreatic and salivary amylase rapidly disappeared from the serum in a monoexponential fashion with a mean serum half-time of approximately 83 min. Only about 24% of the amylase cleared from the s...

1. Rabbit antisera to purified rat parotid alpha-amylase were prepared. 2. The relationships between rat parotid-gland, pancreatic, serum and liver amylase were investigated by using the antisera in immunodiffusion, immunoelectrophoresis and immunoinhibition experiments. 3. Serum and liver amylase were identical, and very similar to parotid-gland amylase; pancreatic amylase was, however, quite ...

There have been several indications (1, 2) that the amylase found in serum is derived from sources other than the pancreas or salivary glands. For this reason we wished to determine whether any indication of fractionation of serum amylase could be obtained by paper electrophoresis. Although several accounts of the fractionation of amylase in serum are found in the literature, some involving pre...

BACKGROUND Low serum amylase levels may reflect impaired exocrine-endocrine relationship in the pancreas. However, few clinical studies have addressed this issue. Therefore, in this epidemiological study, we investigated whether low serum amylase was associated with the pathogenesis of impaired insulin action: metabolic syndrome (MetS) and diabetes. RESEARCH DESIGN AND METHODS Serum amylase, ...

Serum amylase activity was measured in 15 normal persons and in 60 liverdisease patients. Impairment of liver was assessed by serum bilirubin and thymol turbidity values. Most of the patients had serum amylase values that were well below the normal limits. Amylase activities were related to the degree of liver dysfunction, and serum amylase decreased as the bilirubin and turbidity values increa...

In this rapid quantitative assay for pancreatic alpha-amylase (EC 3.2.1.1) in serum, we precipitate salivary amylases by 10-min incubation with monoclonal anti-salivary amylase antibody immobilized on particles of polyvinylidene fluoride. We then centrifuge the serum mixture and measure the pancreatic amylase activity remaining in the supernate by a kinetic method. The assay requires 50 microL ...

Tetracycline and related compounds are used extensively as broad spectrum antibiotics in the treatment of bacterial infections in ruminants. Tetracycline may cause acute pancreatitis which may result in increased serum amylase activity. However, it has been shown that administration of oxytetracycline in human results in decrease serum amylase activity. In this study changes in serum amylase ac...