background: the aim of this study was to assess the performance of antigen b (agb) isolated from different echinococcus granulosus intermediate hosts and from different cyst locations for immu­nodiagnosis of human cystic echinococcosis (ce). methods: hydatid cyst fluids were collected from lung and liver cysts of sheep, liver cysts of goats, lung cysts of camels and cattle, and liver cysts of h...

background: we have previously reported that a 31 kda protein was screened from the excretory-secretory (es) proteins of tichinella spiralis muscle larvae (ml) by immunoproteomics using early infection sera, and the gene encoding a 31 kda protein from t. spiralis was cloned and expressed in an e. coli expression system. in this study, the recombinant 31 kda antigens were used for detection of a...

Context: Microsporidiosis is an emerging disease in both immunocompetent and immunocompromised patients. Diagnosis of human microsporidiosis challenging. Parasitological tests using staining methods have limitations for detecting spores stool samples. The performance molecular diagnosing satisfactory, but these are expensive time-consuming. Serodiagnosis the detection antibodies insufficient sp...

In this report we demonstrate the utility of an monoclonal antibody inhibition enzyme-linked immunosorbent assay based on the Mycobacterium leprae 35-kDa protein, purified from the rapidly growing host Mycobacterium smegmatis, for the serodiagnosis of multibacillary leprosy. The assay proved highly specific (97.5%) and sensitive (90%) and compared favorably with two other established methods ro...

ELISA and RIFI are currently used for serodiagnosis of canine visceral leishmaniasis (CVL). The accuracy of these tests is controversial in endemic areas where canine infections by Trypanosoma cruzi may occur. We evaluated the usefulness of synthetic peptides that were selected through phage display technique in the serodiagnosis of CVL. Peptides were chosen based on their ability to bind to Ig...

The serologic assay is an important tool in the diagnosis of leishmaniasis. One of the most commonly used tests is enzyme-linked immunosorbent assay (ELISA). Since total Leishmania promastigotes are used as antigen in the routine assay, false-positive reactions are frequent due to cross-reaction with sera from other diseases, mainly Chagas' disease. Therefore, an antigen that determines less cr...

Two evaluations of the automated fluorescent treponemal antibody (AFTA) test for the serodiagnosis of syphilis are described. The results of AFTA and manually performed fluorescent treponemal antibody-absorption (FTA-ABS) tests were compared on serum samples from clinically defined donor groups, and the reproducibility of each procedure was studied. Significant improvement of AFTA test results ...

Genes for flagellin A (FlaA) proteins from European borrelial strains of Borrelia burgdorferi sensu stricto, B. afzelii, and B. garinii were cloned and sequenced. An identity of 92 to 93% was observed in the flaA sequences of the different species. Polyhistidine-tagged recombinant FlaA (rFlaA) proteins were produced in Escherichia coli and used as antigens in Western blotting (WB) and enzyme-li...

The need for a rapid and accurate test for the serodiagnosis of syphilis has led to the following investigations. The S.S. test, as described by Seiler, has been used at this clinic for 1,326 cases, which have been cross-checked by other serological reactions performed by outside laboratories. In all cases in which differences were found, blood investigations were repeated. Many sera were dupli...