IFN-gamma mRNA expression was evaluated in nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT-PCR and the sensitivity of these methods was compared. A significant correlation was found between quantitative competitive and semiquantitative RT-PCR in samples of both HIV-seronegative (P = 0.004...

Peppermint has considerable commercial value and widely cultivated for essential oil production, especially menthol. The aim of this study was to determine the quantitative expression of pulegone reductase (pr), menthofuran synthase (mfs) and limonene synthase (ls) genes in menthol biosynthesis pathway in Mentha x piperita , using semiquantitative RT-PCR analysis and evaluating menthol producti...

peppermint has considerable commercial value and widely cultivated for essential oil production, especially menthol. the aim of this study was to determine the quantitative expression of pulegone reductase (pr), menthofuran synthase (mfs) and limonene synthase (ls) genes in menthol biosynthesis pathway in mentha x piperita , using semiquantitative rt-pcr analysis and evaluating menthol producti...

AIM To determine if beta defensins are expressed in the anterior segment of the eye and to determine the temporal pattern of expression using a real time semiquantitative reverse transcription polymerase chain reaction (RT-PCR). METHODS Ocular tissue (corneal epithelium, conjunctiva, iris, and lens capsule) was collected from 23 patients undergoing surgery. Serial corneal or conjunctival impr...

Attempts are frequently made to semiquantitate mRNA as a means of circumventing the laborious and time-consuming process of quantitation that is inherent in the use of competitor templates. However, semiquantitative approaches present the risk of generating non-reproducible data due to tube-to-tube variability and/or misinterpretation of quantities of product being generated during the plateau ...

Abstract: iNOS is inducible by a variety of factors related to inflammation and referred to as inducible NOS(iNOS). It is regulated at the level of gene expression; once expressed, it produces NO at a high rate. iNOS gene-expression profiling is an important tool in understanding molecular markers of the responses of cells and tissues to external factors. In this article a semiquantitative reve...