In this work, performance of hollow fiber membrane photobioreactor (HFMPB) on the growth of Dunaliella Salina (G26) at various aeration rates (0.1 and 0.2 VVm) and medium re-circulation flow rates (500 and 1000 mL/h) were studied. Cultivation was carried out at both batch and semi-continuous modes in HFMPBs containing neat and hydrophilized in-house fabricated poly ethylene (PE) membranes at fi...

In this work we present a device specifically designed to study microbial growth with several applications related to environmental microbiology and other areas of research as astrobiology. The Automated Measuring and Cultivation device (AMC-d) enables semi-continuous absorbance measurements directly during cultivation. It can measure simultaneously up to 16 samples. Growth curves using low and...

From an engineering point of view, the effect of light intensity distribution on the stability of growth rate should be taken into account in designing effective photobioreactors and sustaining stable growth rates. In the experiments described here, in order to keep operational parameters at an almost constant level, a semi-continuous culture method was developed for cultivation of photosynthet...

in this paper, we have dened and studied a generalized form of topological vectorspaces called s-topological vector spaces. s-topological vector spaces are dened by using semi-open sets and semi-continuity in the sense of levine. along with other results, it is provedthat every s-topological vector space is generalized homogeneous space. every open subspaceof an s-topological vector space is ...

Vero cells were grown adherent to microcarriers (Cytodex 1; 3 g L(-1)) using animal component free media in stirred-tank type bioreactors. Different strategies for media refreshment, daily media replacement (semi-batch), continuous media replacement (perfusion) and recirculation of media, were compared with batch cultivation. Cell densities increased using a feed strategy from 1×10(6) cells mL(...

BACKGROUND The filamentous ascomycete Aspergillus niger is used in many industrial processes for the production of enzymes and organic acids by batch and fed-batch cultivation. An alternative technique is continuous cultivation, which promises improved yield and optimized pipeline efficiency. RESULTS In this work, we have used perfusion (retentostat) cultivation to validate two promoters that...

We put forward a revised definition of stably compact spaces which allows us to show their equivalence with Nachbin’s compact ordered spaces in an entirely elementary fashion. We then exhibit some constructions for stably compact spaces which apparently have not appeared in the literature before. These constructions allow us to show that the set of (sub-)probability valuations can be equipped w...

One important aim in cell culture-based viral vaccine and vector production is the implementation of continuous processes. Such a development has the potential to reduce costs of vaccine manufacturing as volumetric productivity is increased and the manufacturing footprint is reduced. In this work, continuous production of Modified Vaccinia Ankara (MVA) virus was investigated. First, a semi-cont...