In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucro...

Artificial insemination (AI) is one of the first reproductive biotechnologies has been established and developed in the pig production system. In most case, liquid stored semen or fresh semen is used for AI in commercial swine herds (Wagner and Thibier, 2000). The use of FT boar semen for AI is limited due to the low fertility outcomes compared to extended fresh semen (Johnson et al., 2000; Wag...

Cryopreservation has the capacity to extend spermatozoa's lifespan and viability. In addition, the semen samples can be collected, preserved and stored or sent to distant locations and still be used long after the death of the semen donor. In this study for the vitrification of dog sperm (fresh and swum-up sperm), different cryopreservation mediums on the basis of glycerol, milk and egg yolk we...

The genetic improvement of agriculturally important species and disease control are of fundamental importance to the success of a sustainable agri-food industry. In this sense, artificial insemination (AI) is arguably the most important tool contributing to the advancement of modern animal production. Through AI, 1 ejaculate from a genetically superior male can be used to impregnate multiple fe...

BACKGROUND Adult cancer patients are routinely offered pre-treatment sperm cryopreservation. However, only recently has the welfare of adolescent cancer sufferers gained momentum, including their infertility, and unsurprisingly relatively little is known about their semen quality and feasibility of cryopreservation. METHODS AND RESULTS A total of 238 adolescent cancer patients referred to our...

The present paper highlights aspects of the cryopreservation of boar semen, a species with particular large, fractionated ejaculates, and a cumbersome cryotechnology that had prevented its commercial application. With the dramatic increase of use of liquid pig semen for artificial breeding over the past decade, developments on cryopreservation alongside the routine use of stud boar semen for AI...

background: human sperm cryopreservation has proven to be very valuable. cryopreservation has effects on function, morphology and percentage of fertilization capacity of human sperm. also, it has been revealed that cryopreservation has a role in sperm dna fragmentation and infertility. in this study, viability, motility, dna fragmentation and for the first time, intracellular nitric oxide asses...

Although the development of semen cryopreservation in the African elephants (Loxodonta africana) has been accomplished, effective procedures for cryopreservation of Asian elephant (Elephas maximus) spermatozoa have not been established. In the present study, we investigate the freezing methods for conservation of Asian elephant spermatozoa under field conditions and identify the most suitable f...

Semen cryopreservation and artificial insemination (AI) are potentially valuable methods for supporting the breeding management of endangered species like the Asian elephant. Cryopreservation of Asian elephant semen has however proven problematic with respect to maintenance of both adequate semen quality and fertility post-thaw. In this study, nine ejaculates from three adult bulls were used to...

OBJECTIVE To review sperm cryopreservation usage rates, corresponding reproductive outcomes, and the current situation in our locality. DESIGN Retrospective case series. SETTING Assisted Reproductive Technology Unit of the Department of Obstetrics and Gynaecology, Prince of Wales Hospital and the Chinese University of Hong Kong. PARTICIPANTS There were 130 Chinese male patients who underw...