Stevia rebaudiana is a herbaceous perennial plant with great global demand due to its beneficial steviol glycosides(SGs) content. Current conventional breeding technique unable cater the need for more S. planting materials. Therefore, an improved in vitro shoot regeneration protocol was developed by using chitosan. The highest fresh weight of (0.586 ± 0.176 g/explant), dry (0.056 0.02 g/explant...

Pieces of tissue were dissected from early chick and quail embryos (Stages XIII and XIV of Eyal-Giladi & Kochav, 1976; and stages 3-5 of Hamburger & Hamilton, 1951). These tissues were taken from three different regions of the early embryos, and from eight different regions of the older ones, and were derived mainly from the lower layer. Epiblast tissues were also used. The experiments were des...

A 28-year-old female presented with a palpable mass lesion on the superonasal aspect of her right globe and she had a progressive diplopia. She had a scleral encircling surgery with a Miragel explant (MIRA, Waltham, Mass, USA) for the tractional retinal detachment associated with pars planitis 9 years previously. On examination, she revealed restricted eye movements of her right eye. The magnet...

BACKGROUND Scleral buckling is still a common procedure to repair rhematogenous retinal detachment, and acute or chronic infection of the scleral explant is rare. We report an intractable case of acute scleral explant infection by Pseudomonas aeruginosa. CASE A 36-year-old man suffered from acute scleral explant infection by P. aeruginosa forty-eight hours after scleral buckling for rhegmatog...

Efficient procedures are outlined for plant regeneration through direct shoot bud formation and indirect organogenesis through callus formation in Ornithogalum virens Lindl using bulb scale as explant. Murashige and Skoog’s (MS) medium containing 1 mg/L (5.4mM) NAA and 2 mg/L (4.4mM) BA was most effective in direct induction of shoot buds from explant. Callus cultures were raised from the bulb ...

An efficient procedure for in vitro shoot production of the medicinal plant sundew (Drosera rotundifolia L.) was developed. Of three cytokinins tested, zeatin (ZEA) at a concentration of 2 μM resulted in the formation of large numbers of adventitious shoots on leaf explants. A larger size of the small shoots was achieved by combining a 2-weeks preculture with ZEA (step 1: shoot induction) with ...

Testing cardiac gene and cell therapies in vitro requires a tissue substrate that survives for several days in culture while maintaining its physiological properties. The purpose of this study was to test whether culture of intact cardiac tissue of neonatal rat ventricles (organ explant culture) may be used as a model to study gene and cell therapy. We compared (immuno) histology and electrophy...

Fritillaria meleagris is a horticulturally and medicinally valuable bulbous plant that requires period of low temperatures for proper growth flowering. Since conventional methods propagation are ineffective very slow, tissue culture techniques offer an integrated approach to mass production this geophyte. In study, we investigated the effects various auxin–cytokinin combinations on different mo...