Recent developments in studies of sperm-mediated gene transfer (SMGT) now provide solid ground for the notion that sperm cells can act as vectors for exogenous genetic sequences. A substantive body of evidence indicates that SMGT is potentially useable in animal transgenesis, but also suggests that the final fate of the exogenous sequences transferred by sperm is not always predictable. The ana...

The generation of transgenic animals by conventional transgenic protocols is cumbersome and not very efficient. To improve the efficiency of obtaining transgenic animals, different groups have attempted to genetically modify spermatozoa and these technologies are collectively referred to as sperm mediated gene transfer (SMGT). SMGT technologies involve the modification of either spermatozoa or ...

A large number of hDAF transgenic pigs to be used for xenotransplantation research were generated by using sperm-mediated gene transfer (SMGT). The efficiency of transgenesis obtained with SMGT was much greater than with any other method. In the experiments reported, up to 80% of pigs had the transgene integrated into the genome. Most of the pigs carrying the hDAF gene transcribed it in a stabl...

Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described the SMGT procedure in a small animal model, with high efficiency reported in the mouse. In addition...

The novel strengthen-modified grinding technique (SMGT) treatment was carried out on 30CrMnSiA bearing steels to investigate the effect of jet pressure (0.2–0.6 MPa) and angle (30–90°) its surface micromorphology, microstructure, mechanical properties. results show that, under compound effects impact steel beads abrasive powder micro-cutting, specimens treated by SMGT has a microstructure with ...

Aim: To investigate the potential of botulinum toxin A for treating hyperlacrimation. Methods: Three patients with unilateral symptoms of hyperlacrimation (diagnosed as “crocodile tearing”) and one patient with a submandibular salivary gland transplant (SMGT) were studied. Tear production was quantified in the resting and stimulated (chewing or following exercise) state, using Schirmer’s test a...

AIM To investigate the potential of botulinum toxin A for treating hyperlacrimation. METHODS Three patients with unilateral symptoms of hyperlacrimation (diagnosed as "crocodile tearing") and one patient with a submandibular salivary gland transplant (SMGT) were studied. Tear production was quantified in the resting and stimulated (chewing or following exercise) state, using Schirmer's test a...

OBJECTIVES The Streptomyces phage phiC31 integrase offers a sequence-specific method of transgenesis with a robust long-term gene expression. PhiC31 has been successfully developed in a variety of tissues and organs for purpose of in vivo gene therapy. The objective of the present experiment was to evaluate PhiC31-based site-specific transgenesis system for production of transgenic bovine embry...