The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found i...

It has been known for decades that microbial fermentation within the rumen is critical to postnatal rumen epithelial growth and maturation in ruminants, but the underlying mechanism is largely unknown. In this study, we determined the effect of rumen fluid, which should contain all products from rumen fermentation, on growth of rumen epithelial cells in vitro. Addition of 10% rumen fluid from c...

In this study, the kinetic of biogas production was studied by performing a series laboratory experiment using rumen fluid of animal ruminant as inoculums. Cattle manure as substrate was inoculated by rumen fluid to the anaerobic biodigester. Laboratory experiments using 400 ml biodigester were performed in batch operation mode. Given 100 grams of fresh cattle manure was fed to each biodigester...

an in vitro experiment was conducted to study the effect of non-fiber carbohydrates (nfc) on ruminaldisappearance kinetics of dry matter (dm) and neutral detergent fiber (ndf) of alfalfa hay, wheat bran andunmolassed sugar beet pulp. non-supplemented or nfc supplemented samples [70 mg/g dm of each feedsample as sucrose (su) or starch (st) or su + st as 1:1] were incubated in bottles containing ...

Chilling of whole rumen contents prior to preparation of strained rumen fluid enriched with particle-associated microorganisms resulted in rumen inoculum with the highest microbial dry weight, total bacterial counts, and degradation rates for casein and soybean meal. Strained rumen fluid, whole rumen contents blended with strained rumen fluid, chilled strained rumen fluid, and strained rumen fl...

Assessment of the rumen fluid is an ancillary examination technique used in the clinical examination both of the individual bovine patient and at population level. The specific function and structure of the rumen makes ruminants different from other animals. Therefore, it logical to assess the rumen in detail when confronted with a bovine patient. Assessment of the rumen fluid is essential to a...

This study was conducted to investigate the effect of feed types on Ochratoxin A (OTA) degradation by Korean native goats. Rumen fluid from canulated goats fed whole roughage or 50% roughage served as a source of micro-organisms. Experiments were undertaken i) to investigate OTA degradation ability in a 2×4 factorial arrangement with different feed types (100% roughage vs. 50% roughage) and rum...

Biogas productions of cattle manure using rumen fluid inoculums were determined using batch anaerobic digesters at mesophilic temperatures (room and 38.5 C). The aim of this paper was to analyze the influence of rumen fluid contents on biogas yield from cattle manure using fluid rumen inoculums. A series of laboratory experiments using 400 ml biodigester were performed in batch operation mode. ...

the aim of this study was to investigate the fermentation characteristics and cellulolytic enzymes activities of dromedary camels rumen anaerobic fungi in fed with cultivated and pasture forages. hence, the inoculant of rumen anaerobic fungi was prepared by using specific medium and the gas production, digestibility and enzyme activity of rumen fungi were determined on the basis of a 2 × 2 fact...

Intake and excretory patterns, N and mineral balance, rumen fluid characteristics and acid-base status were measured for 15 days in five nonfistulated and six rumen-fistulated wethers. The animals were placed in metabolism crates and fed alfalfa hay from day 1 through 5, a 65% concentrate diet from day 6 through 10 and an 85% concentration diet from days 11 through 15. All diets were pelleted a...