Background: Fusion of the last two enzymes in pyrimidine biosynthetic pathway inverse order by having COOH-terminal orotate phosphoribosyltransferase (OPRT) and NH 2 -terminal orotidine 5′-monophosphate decarboxylase (OMPDC), as OPRT-OMPDC, has been described many organisms. Objective: The study aimed to select optimum host cell temperature for expressing recombinant fusion OMPDC-OPRT enzymatic...

BACKGROUND AND OBJECTIVES Clostridium perfringens, a Gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. C. perfringens is subdivided into five types A to E based on its four major alpha, beta, epsilon and iota toxins. The aim of the present study was cloning and expression of C. perfringens type D vaccine strain epsilon ...

The OCTN subfamily includes OCTN1, 2, and 3 which are structurally and functionally related. These transporters are involved in maintenance of the carnitine homeostasis, which is essential in mammals for fatty acid β-oxidation, VLDL assembly, post-translational modifications, and other essential functions. Indeed, defects of these transporters lead to severe pathologies. OCTN1 and OCTN2 are exp...

ricin, the toxic lectin extracted from the castor bean plant (ricinus communis), consists of an a chain (rta) and a b chain (rtb). anti-a chain abs and anti-b chain abs can neutralize toxins in vivo and in vitro via blocking the binding of the toxin to the cell. also, rtb protein is able to serve as an antigen deliver to the mucosal immune system and act as an immunoadjuant. here, the genomic d...

CPS-1 is a subclass B3 metallo-β-lactamase from a Chryseobacterium piscium isolate collected from soil, showing 68% amino acid identity to the GOB-1 enzyme. CPS-1 was overproduced in Escherichia coli Rosetta (DE3), purified by chromatography, and biochemically characterized. This enzyme exhibits a broad-spectrum substrate profile, including penicillins, cephalosporins, and carbapenems, which ov...

BACKGROUND Escherichia coli (E. coli) is the most extensively used host for the production of recombinant proteins. However, most of the eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. Reteplase as a highly disulfide-bonded recombinant protein is an example of difficult to express protein in E. coli. METHODS In this s...

The scorpion has been extensively used in various pharmacological profiles or as food supplies. The exploration of scorpion venom has been reported due to the presence of recombinant peptides. rBmαTX14 is an α-neurotoxin extracted from the venom gland of the East Asian scorpion Buthus martensii Karsch and can affect ion channel conductance. Here, we investigated the functions of rBmαTX14 using ...

background and objectives: clostridium perfringens, a gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. c. perfringens is subdivided into five types a to e based on its four major alpha, beta, epsilon and iota toxins. the aim of the present study was cloning and expression of c. perfringens type d vaccine strain epsilon...

Using the DNA recombination technique, a new bacterial strain Escherichia coli DAC-22 was derived, whose cells are able to carry out heterologous expression of Bacillus thuringiensis diadenylate cyclase – enzyme catalyzing reaction adenosine-5′-triphosphate (ATP) transformation into cyclic 3′,5′-diadenylate (cyclo-di-AMP). To derive strain, E. “Rosetta (DE3) pLysS” were originally used as recip...