Both phage display and ribosome display systems can be used effectively to isolate large numbers of sequence-diverse antibodies from naı̈ve libraries. Once leads have been identified the same display technologies can be used to engineer the characteristics of the drug candidates to yield the desired in vivo efficacy. Ribosome display has a number of advantages over phage display resulting in sig...

Objective(s): Tuberculosis (TB) is still one of the problematic infectious diseases in developing countries, especially in Iran. In the present study, we applied ribosome display technique to select single chain variable fragments (scFvs) specific for the 6-kDa early secretory antigenic target (ESAT-6) antigen of Mycobacterium tuberculosis from a mouse scFv library. Materials and Methods: The g...

Ribosome display is an in vitro evolution technology for proteins. It is based on in vitro translation, but prevents the newly synthesized protein and the mRNA encoding it from leaving the ribosome. It thereby couples phenotype and genotype. Since no cells need to be transformed, very large libraries can be used directly in selections, and the in vitro amplification provides a very convenient i...

Ribosome display is a cell-free system for the in vitro selection of proteins and peptides from large libraries. It uses the principle of coupling individual nascent proteins (phenotypes) to their corresponding mRNA (genotypes), through the formation of stable protein–ribosome–mRNA (PRM) complexes. This permits the simultaneous isolation of a functional nascent protein, through affinity for a l...

Over the last ten years phage display has been employed for the potency optimisation of many antibodies which have progressed into clinical development. More recently, ribosome display has emerged as a powerful alternative to phage display for antibody optimisation. In this study we have analysed in-house data from the optimisation of 30 antibodies by phage and/or ribosome display to compare th...

Combinatorial approaches in biology require appropriate screening methods for very large libraries. The library size, however, is almost always limited by the initial transformation steps following its assembly and ligation, as other all screening methods use cells or phages and viruses derived from them. Ribosome display is the first method for screening and selection of functional proteins pe...