نتایج جستجو برای: RhAA gene

تعداد نتایج: 1141368  

Journal: :Applied and environmental microbiology 2003
Paloma Manzanares Margarita Orejas José Vicente Gil Leo H De Graaff Jaap Visser Daniel Ramón

The Aspergillus aculeatus rhaA gene encoding an alpha-L-rhamnosidase has been expressed in both laboratory and industrial wine yeast strains. Wines produced in microvinifications, conducted using a combination of the genetically modified industrial strain expressing rhaA and another strain expressing a beta-glucosidase, show increased content mainly of the aromatic compound linalool.

2016
Senthil Kumar Velusamy Vandana Sampathkumar Dipti Godboley Daniel H. Fine

Leukotoxin (Ltx) is a prominent virulence factor produced by Aggregatibacter actinomycetemcomitans, an oral microorganism highly associated with aggressive periodontitis. Ltx compromises host responsiveness by altering the viability of neutrophils, lymphocytes, and macrophages. Previously, we developed a Rhesus (Rh) monkey colonization model designed to determine the effect of virulence gene mu...

Journal: :Applied and environmental microbiology 2001
P Manzanares H C van den Broeck L H de Graaff J Visser

Two proteins exhibiting alpha-L-rhamnosidase activity, RhaA and RhaB, were identified upon fractionation and purification of a culture filtrate from Aspergillus aculeatus grown on hesperidin. Both proteins were shown to be N glycosylated and had molecular masses of 92 and 85 kDa, of which approximately 24 and 15%, respectively, were contributed by carbohydrate. RhaA and RhaB, optimally active a...

2014
Ester Pardo Margarita Orejas

BACKGROUND Various plant-derived substrates contain L-rhamnose that can be assimilated by many fungi and its liberation is catalyzed by α-L-rhamnosidases. Initial data obtained in our laboratory focussing on two Aspergillus nidulans α-L-rhamnosidase genes (rhaA and rhaE) showed α-L-rhamnosidase production to be tightly controlled at the level of transcription by the carbon source available. Whi...

2012
Juan A Tamayo-Ramos Michel Flipphi Ester Pardo Paloma Manzanares Margarita Orejas

BACKGROUND Little is known about the structure and regulation of fungal α-L-rhamnosidase genes despite increasing interest in the biotechnological potential of the enzymes that they encode. Whilst the paradigmatic filamentous fungus Aspergillus nidulans growing on L-rhamnose produces an α-L-rhamnosidase suitable for oenological applications, at least eight genes encoding putative α-L-rhamnosida...

Khadijeh Razavi Mahsa Aminsalehi, Maoumeh Zeinipour Parissa Jonoubi,

Rose is the most favorite cut flowers all over the world. Production of high-quality flowers, prevention, and delay of flower senescence, is a major goal in floriculture. Now a day, biotechnological approaches have been used to improve ornamental attributes. Tissue culture and genetic transformation appear to offer valuable advancements for operating floral characteristics. In this study, after...

2014
Juan Antonio Tamayo-Ramos Margarita Orejas

Background: Engineered fungi are attractive platforms for the production of plant cell wall hydrolytic enzymes which, among other biotechnological applications, are required for the efficient conversion of biomass to glucose and other fermentable sugars. As a fungal model system, Aspergillus nidulans provides genetic tools that are of relevance in this context and potentially applicable to indu...

Journal: :Journal of bacteriology 1987
Y M Chen J F Tobin Y Zhu R F Schleif E C Lin

Dissimilation of L-fucose as a carbon and energy source by Escherichia coli involves a permease, an isomerase, a kinase, and an aldolase encoded by the fuc regulon at minute 60.2. Utilization of L-rhamnose involves a similar set of proteins encoded by the rha operon at minute 87.7. Both pathways lead to the formation of L-lactaldehyde and dihydroxyacetone phosphate. A common NAD-linked oxidored...

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