نتایج جستجو برای: Restriction fragment length polymorphism
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conclusions using the pcr-rflp method, three distinctive h. pylori caga genotypes were detected in antral biopsies. genotype i, which was predominant among the isolates, was significantly associated with gastritis. however, the data showed that caga genotype iii may play a role in duodenitis and duodenal ulcers in patients infected with h. pylori. patients and methods a total of 161 gastric bio...
background: molecular components of the dopamine receptor (drd3) play an important role in the pathophysiology of schizophrenia (scz). previous studies have demonstrated an association between the drd3 ser9gly polymorphism and scz but the results have been inconclusive. method: in this study, we investigated this controversial association between the ser9gly (a/g) polymorphism and scz using mal...
background coronary artery disease (cad) is the most common cause of death worldwide. mef2a directly regulates target genes in the process of muscle development. this gene product is a transcription factor. mef2a protein in homodimer or heterodimer forms binds to a/t-rich cis elements with conserved sequence in promoter, regulator, and enhancer of many genes, which are determining in evolution ...
conclusions the results revealed that the strains under study could be epidemically related. it seems that an alternative subtyping method is needed to study the relationship among clinical s. sonnei strains and their transmission. here, we reported for the first time, two strains of s. sonnei with a different pcr-rflp pattern for ipah gene. results all shigella isolates were positive for both ...
conclusions several previous studies used pcr-rflp to genotype hbv; however, we showed the high risk to obtain atypical profiles, especially in advanced stages of chronic infection, with as results difficulties to genotype the virus. these profiles resulted from the accumulation of mutations during natural course of infection resulting in a modification in restriction sites for enzymes. so, we ...
مقدمه: candidiasis منتشره، دیابت، حاملگی، مصرف بی رویه ی آنتی بیوتیک های وسیع الطیف، عوامل مستعد کننده ی candidiasis دستگاه ادراری می باشند. شیوع عفونت با گونه های غیر candida albicans رو به افزایش است. هدف از انجام این مطالعه، بررسی میزان شیوع گونه های candida مسبب کاندیدوری در شهر اصفهان با استفاده از روش polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) بود. روش ه...
conclusions the results of the present study, compared to the previous studies, showed a significantly lower prevalence of cutaneous candidiasis. among candida species, c. albicans was still the most common infectious agent isolated from clinical samples. shifting toward non-albicans candida species was not confirmed in this study. in addition, our study revealed that there is a direct correlat...
identification of clinically significant nocardia species is essential for the definitive diagnosis, predict antimicrobial susceptibility, epidemiological purposes, and for an effective treatment. conventional identification of nocardia species in routine medical laboratories which is based on phenotypic (cellular morphology, colonial characteristics), biochemical and enzymatic...
Blackwood et al. (1) assert that terminal restriction fragment length polymorphism (T-RFLP) data cannot be used to estimate the species level diversity of bacterial communities. While I do not argue with their analytical techniques, nor the interpretation of their results, their conclusion is essentially moot as T-RFLP techniques are rarely used (nor should they be used) to study the diversity ...
A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method wa...
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