Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...

BACKGROUND: Fat-tailed sheep breeds have a  unique  ability  to  tolerate  periods  of  negative  energy  balance  due to seasonal changes in feed availability. This ability is attributed to presence  of  fat-tail  as  a  body  energy  reserve, however the exact underlying  mechanisms  controlling  the  response  of adipose  tissue  depots  to variations in energy balance in fat-tailed breeds a...

objective: understanding gene expression variations by using rna transcript analysis methods during hepatic viral protein interactions with the ifn pathway in hepatic cell lines has recently gained importance. one of the most powerful techniques in gene expression quantification is quantitative real-time rt-pcr. reference genes used as normalizer in this method may be affected across various ex...

esophageal squamous cell carcinoma (escc) is a deadly cancer with poor prognosis. in this regard, early diagnosis is of vital importance to cure the tumor in its early stages. novel cancer diagnostic and therapeutic approaches have been recently introduced based on micrornas (mirnas). also, accurate normalization using appropriate reference genes is a critical step in mirna expression studies. ...

objective: in spite of accumulating information about pathological aspects of sulfur mustard (sm), the precise mechanism responsible for its effects is not well understood. circulating micrornas (mirnas) are promising biomarkers for disease diagnosis and prognosis. accurate normalization using appropriate reference genes, is a critical step in mirna expression studies. in this study, we aimed t...

RNA-sequencing is a new method of transcriptome characterization of organisms. Based on identity and relatedness, there are large genetic variations among different cattle breeds. The goal of the current study was to sequence the transcriptome of Guilan native cow and compare with available reference genome using RNA-sequencing method. Blood samples were collected from 14 Guilan native cows and...

throughout the present study the effect of using markers close to genes affecting a trait, ascompared with the use of all the markers of a genome, and also the effect of on increase in thenumber of reference groups, on the accuracy of a genetic evaluation, was investigated. genomicevaluation was done using blup method. the results indicated that use of markers close to genesaffecting a trait an...