objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Avian Pasteurella multocida is the causative agent of fowl cholera, a disease much affecting the poultry industry. In order to study the efficacy of the recombinant subunit vaccine constructed with ptfA gene of avian P. multocida, the ptfA gene fragment amplified by PCR from avian P. multocida was cloned into the prokaryotic expression vector pET32a and the recombinant plasmid pET32a-ptfA was o...

Objective(s): Despite treatment with antibiotics and vaccination with BCG, tuberculosis (TB) is still considered as one of the most important public health problems in the world. Therefore, designing and producing a more effective vaccine against TB seems urgently. In this study, immunogenicity of a fusion protein which consisting or comprising CFP-10 from Mycobacterium tuberculosis and the Fc-...

objective(s): despite treatment with antibiotics and vaccination with bcg, tuberculosis (tb) is still considered as one of the most important public health problems in the world. therefore, designing and producing a more effective vaccine against tb seems urgently. in this study, immunogenicity of a fusion protein which consisting or comprising cfp-10 from mycobacterium tuberculosis and the fc-...

objectives: enterotoxigenic escherichia coli (etec) is one of the most common agents of diarrhea among other bacterial agents. designing and producing vaccine against these bacteria is one of the major purposes of world health organization (who). due to presence of diverse clones of etec strains in the world, the use of global vaccines for etec infection is controversial. b subunit of heat labi...

Background: Enterotoxigenic Escherichia coli (ETEC) strains are the major causes of diarrheal disease in humans and animals. Colonization factors and enterotoxins are the major virulence factors in ETEC pathogenesis. For the broad-spectrum protection against ETEC, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. Methods: A fusion protein is composed of a...

background and objectives: brucellosis is an important health problem in developing countries and no vaccine is available for the prevention of infection in humans. because of clinically infectious diseases and their economic consequences in human and animals, designing a proper vaccine against brucella is desirable. in this study, we evaluated the immune responses induced by a designed recombi...

background: enterotoxigenic escherichia coli (etec) strains are the major causes of diarrheal disease in humans and animals. colonization factors and enterotoxins are the major virulence factors in etec pathogenesis. for the broad-spectrum protection against etec, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. methods: a fusion protein is composed of a...