background: there are different activating salt solutions for sperm induction of rainbow trout (oncorhynchus mykiss) under artificial spawning condition, although some uncertainties are associated with the performance. objectives: this study was conducted to consider the effects of different concentration of calcium and magnesium ions and change of ph in the billard salt solution on sperm motil...

objective: to study the morphology ofgonads and sperm ducts of sex reversed male rainbow trout after administration of i 7-cr- methyl testosterone. animals: rainbow trout, oncorhynchus mykiss. procedure: l7-alpha-methyl testosterone (17-alpha-mt) was administered to rainbow trout in 5 treafinents as follows: i) rwo times immersion of eyed eggs and yolk sac alevins in 250 pg/l bath with 2 hours ...

Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in...

Polymorphism. A variable number of tandem repeat (VNTR; Nakamura et al., 1987) polymorphism was detected in rainbow trout (Oncorhynchus rnykiss Walbaum) using BgZII. The probe, RW9401, was isolated from a genomic DNA cosmid library developed from the RTG-2 cell line (Wolf and Quimby, 1962). Method of Detection. Nylon filters containing rainbow trout genomic DNA digested with BgZII were prehy...

Polymorphism. A variable number of tandem repeat (VNTR; Nakamura et al., 1987) polymorphism was detected in rainbow trout (Oncorhynchus mykiss Walbaum) using MspI and BgZII. The probe, RVF9303, was isolated from a genomic DNA cosmid library developed from the RTG-2 cell line (Wolf and Quimby, 1962). Method of Detection. Nylon filters containing rainbow trout genomic DNA digested with MspI o...

The effects of different extenders and storage periods on the motility and fertilization rate of rainbow trout (Oncorhynchus mykiss) semen were evaluated after short-term storage. Semen was collected from anesthetized males by the abdominal massage. After determination of main semen characteristics, the pooled ejaculates were diluted with 4 different extenders at a ratio of 1:3, and stored at 4...

This study was designed to compare the performance of the kits Diff-Quick, Hemacolor and Spermac for staining the spermatozoa of rainbow trout. Automated sperm morphology analysis (ASMA) was performed using two image analysis programs to determine the sperm measurements: head size (length, width, area and perimeter), shape (ellipticity, rugosity, elongation and regularity) and tail length. Diff...

Herein we demonstrated the effect of oxygen and air atmosphere on the success of short-term storage of sperm obtained from rainbow trout (Oncorhynchus mykiss), sterlet (Acipenser ruthenus) and common carp (Cyprinus carpio) diluted in immobilizing media (100 mM NaCl, 40 mM KCl, 3 mM CaCl2, 1.5 mM MgCl2, 50mM Tris, pH 8.2 for salmonid fish, 17 mM NaCl, 1.7 mM KCl, 6 mM NaHCO3, 0.5 mM CaCl2, 0.5 m...

Rainbow trout, oncorhynchus mykiss is one of the most important cold water fishes in Iran. For successful artificial propagation of rainbow trout, the quality and quantity of brooders and sexual materials are key factors. In the present study, we investigated the age-dependent changes of reproductive efficiency. For this purpose, the brooders were divided into age classes and then three age gro...