نتایج جستجو برای: RNA-Seq
تعداد نتایج: 256145 فیلتر نتایج به سال:
given the importance of transcriptome analysis in various biological studies and considering thevast amount of whole transcriptome sequencing data, it seems necessary to develop analgorithm to assemble transcriptome data. in this study we propose an algorithm fortranscriptome assembly in the absence of a reference genome. first, the contiguous sequencesare generated using de bruijn graph with d...
In this study the cis and trans regulatory effect of long non-coding genes (lncRNA) on the expression of genes in fish infected by Viral hemorrhagic septicemia virus (VHS) was investigated using RNA-seq technology. At the end of experimental period (the thirty fifth day), total RNA was extracted from spleen tissue (group treated with virus) and physiological serum (control group) was used to pr...
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از بین نشانگر های به کار رفته در prunus، نشانگر های ریزماهواره مبتنی بر ژنوم، اطلاعات پیرامون فرایند های مرتبط با بخش بندی رویشگاه ها و جمعیت های کوچک، توزیع تنوع آللی، ارایه ی الگو های مدیریت ژرم پلاسم و روابط ژنتیکی در زمینه حفاظت ژنتیکی را بهبود بخشیده است. به علاوه بررسی ترانسکریپتومیک و ژنومیک امکان مطالعه تنوع عملکردی ژن ها، ناشی از عملکرد حاصل از بخش بندی رویشگاه، تنوع میزان مقاومت در بر...
Background: Mammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. However, it is still unclear about the crucial transcriptional network and key hub genes that regulate the proceeding of preimplantation embryos. Materials and Methods: Through single-cell RNAsequencing (RNA-seq) of both human and mouse preimplantation embryos, ...
ObjectiveTo explore the exome and transcriptome characteristics potentially underlying pathogenesis of varicocele (VE).DesignExperimental study cohort study.SettingAcademic research laboratory university-affiliated hospital.Patient(s)Eleven VE patients whose fathers also had VE, plus 151 additional 324 healthy men for variants genotyping; rat model, eight Sprague–Dawley male rats.Intervention(s...
Introduction Cellular senescence (CS) plays a critical role in cancer development, including clear cell renal carcinoma (ccRCC). Traditional RNA sequencing cannot detect precise molecular composition changes within tumors. This study aimed to analyze cellular senescence’s biochemical characteristics ccRCC using single (ScRNA-seq) and traditional (Bulk RNA-seq). Methods Researchers analyzed the ...
Soybean, one of the most important sources of edible oil and protein in the world, is exposed to various environmental biotic and abiotic stresses. These stresses can negatively impact the quality and quantity of soybean production. This study aimed to identify genes that express circular RNAs in response to low phosphorus and nitrogen stresses in soybean roots. Soybean seeds were grown under d...
RNA-sequencing (RNA-seq) allows global gene expression analysis at the individual transcript level. Accurate quantification of transcript variants generated by alternative splicing (AS) remains a challenge. We have developed a comprehensive, nonredundant Arabidopsis reference transcript dataset (AtRTD) containing over 74 000 transcripts for use with algorithms to quantify AS transcript isoforms...
While RNA sequencing (RNA‐seq) has become increasingly popular for transcrip‐ tome profiling, the analysis of the massive amount of data generated by large‐scale RNA‐seq still remains a challenge. RNA‐seq data analyses typically consist of (1) accurate mapping of millions of short sequencing reads to a reference genome, including the identification of splicing events; (2) quantifying expression...
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