RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extracti...

rna extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. although many rna extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. many studies have shown that trizol is an applicable material for the rna extracti...

background: rna interference (rnai) is a phenomenon uses double-stranded rna (dsrna) to specifically inhibit gene expression. the non-specific silencing caused by interferon response to dsrna in mammalian cells limits the potential of utilizing rnai to study gene function. duplexes of 21-nucleotide short interfering dsrna (sirna) inhibit gene expression by rnai. in some organisms, sirna can als...

conclusions the pattern of longitudinally increased liver stiffness measurements efficiently supported clinical decisions for individualized management strategies. repeated transient elastography in real-life clinical practice appears to have a practical role in monitoring liver transplanted patients. background liver transplanted patients need close surveillance for early signs of graft diseas...

................................................................................................................ xix

Dicer is a specialized nuclease that produces RNA molecules of specific lengths for use in gene silencing pathways. Dicer relies on the correct measurement of RNA target duplexes to generate products of specific lengths. It is thought that Dicer uses its multidomain architecture to calibrate RNA product length. However, this measurement model is derived from structural information from a protoz...

Advances in microarray technology have led to the increased demand for techniques appropriate for the analysis of RNA. The two main parameters to be assessed are RNA quality and the quantitation of RNA. Absorbance and fluorescence based measurements are established techniques for RNA quantitation. A new technique for the measurement of RNA is the Agilent 2100 bioanalyzer in combination with the...