Background: The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have devel oped an efficient...

The open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. One possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. Themain purpose of this study was to compare the survival rates of in vitro produced b...

Mice are ideal models for ICSI experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. Unfortunately, it has been extremely difficult. In this study we improved the mouse ICSI method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. The outer pipette ...

the open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. one possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. themain purpose of this study was to compare the survival rates of in vitro produced b...

The disposition of the Anlage fields of the neural plate and the intraembryonic mesoblast in the upper layer of the chicken blastoderm was studied at the primitive streak stage prior to the regression of Hensen's node (stages 5V to 6V, L. Vakaet (1970) Arch. Biol. 81, 387-426). Chicken blastoderms were cultured by New's technique on a mixture of thin egg white and agar. The anterior half of the...

mice are ideal models for icsi experiments because of the ease of culturing eggs/embryos in vitro and the availability of ample genetic information. unfortunately, it has been extremely difficult. in this study we improved the mouse icsi method by using a new holding pipette that was made of two pipettes such that one pipette was pulled and heat merged into the other one. the outer pipette had ...

~~~~~~~~~~~~~~~~~~~~W Mr. N. SHAH, lecturer, Professorial Unit, the Institute of Laryngology and Otology, London W.C.1, writes: Myringotomy and aspiration of fluid from the middle ear is a common practice in otology. Children with fluid (serous or "glue ") are operated on under general anaesthesia with the aid of a Zeiss microscope. A glass suction cannula (see photograph) for aspiration of flu...

Materials and Methods As the polychromasic cells (Key, 1921; Rodan, 1949) and nucleated red cells (Stein, 1948; Rodan, 1949) collect in the buffy layer, a blind study was carried out on the buffy layer from 95 patients, including 11 children under the age of 6 years. A total of 137 slides (no more than two from each patient) was examined. With one exception (an iron deficiency anaemia) all slid...

Quartz capillaries with a 0.3 mm inner diameter and a 0.4 mm outer diameter were purchased from Hilgenberg. They were pulled using the program HEAT = 620, FIL = 0, VEL = 30, DEL = 140, PUL = 150 on a laser-assisted pipette puller (P-2000, Sutter Instruments). A typical pulling time was 0.30 ± 0.03 sec and diameters of the pulled capillaries were in the range of 150−200 nm. All pulled capillarie...

This article presents the results of an experimental model a systemic pseudomonas aeruginosa infection. can be used to develop therapeutic measures suppress infectious disease caused by antibiotic-resistant strains Pseudomonas aeruginosa. For this purpose, dry lyophilised culture (strain name: Aeruginosa No. 453, strain number 190158, obtained from Vishnevsky Institute Surgery. was pre-cultured...