نتایج جستجو برای: Pseudomonas pseudoalkaligene KF707

تعداد نتایج: 53098  

Journal: :Journal of bacteriology 1994
K Furukawa J Hirose S Hayashida K Nakamura

Engineering of hybrid gene clusters between the toluene metabolic tod operon and the biphenyl metabolic bph operon greatly enhanced the rate of biodegradation of trichloroethylene. Escherichia coli cells carrying a hybrid gene cluster composed of todC1 (the gene encoding the large subunit of toluene terminal dioxygenase in Pseudomonas putida F1), bphA2 (the gene encoding the small subunit of bi...

Journal: :Journal of bacteriology 2012
Tania Triscari-Barberi Domenico Simone Francesco Maria Calabrese Marcella Attimonelli Kristen R Hahn Kingsley K Amoako Raymond J Turner Stefano Fedi Davide Zannoni

Pseudomonas pseudoalcaligenes KF707 is a soil polychlorinated biphenyl (PCB) degrader, able to grow both planktonically and as a biofilm in the presence of various toxic metals and metalloids. Here we report the genome sequence (5,957,359 bp) of P. pseudoalcaligenes KF707, which provides insights into metabolic degradation pathways, flagellar motility, and chemotaxis.

Creatinine amidohydrolase(EC 3.5.2.10) catalyzes the reversible conversion of creatinine to creatine. Creatininase in combination with other enzymes is used for detection of creatinine in serum and urine which is of significant value for detection of renal, muscular and thyroid functions. The aim of this study was to produce recombinant creatininase enzyme in E.coli expression system to use it ...

2015
Takahito Watanabe Atsushi Yamazoe Akira Hosoyama Hidehiko Fujihara Hikaru Suenaga Jun Hirose Taiki Futagami Masatoshi Goto Nobutada Kimura Kensuke Furukawa

Pseudomonas toyotomiensis KF710 utilizes biphenyl and degrades polychlorinated biphenyls (PCBs). Here, we report the genome sequence of the KF710 strain, consisting of 5,596,721 bp with 5,155 coding sequences. The biphenyl catabolic genes were almost identical to those of Pseudomonas pseudoalcaligenes KF707, one of the most well-characterized biphenyl-utilizing strains.

2016
Stefano Fedi Tania Triscari Barberi Maria Rosaria Nappi Federica Sandri Sean Booth Raymond J. Turner Marcella Attimonelli Martina Cappelletti Davide Zannoni

A genome analysis of Pseudomonas pseudoalcaligenes KF707, a PCBs degrader and metal-resistant soil microorganism, revealed the presence of two novel gene clusters named che2 and che3, which were predicted to be involved in chemotaxis-like pathways, in addition to a che1 gene cluster. We herein report that the histidine kinase coding genes, cheA2 and cheA3, have no role in swimming or chemotaxis...

Journal: :Environmental Health Perspectives 1995
K Furukawa N Kimura

Biphenyl(BP)-utilizing bacteria, which include both Gram-negative and Gram-positive strains, are ubiquitously distributed in the environment. These bacteria co-metabolically degrade a variety of polychlorinated biphenyl (PCB) congeners to the corresponding chlorobenzoic acids through 2,3-dioxygenation. Certain strains degrade even highly chlorinated PCBs through 3,4-dioxygenation. The ring meta...

2017
Federica Sandri Stefano Fedi Martina Cappelletti Francesco M. Calabrese Raymond J. Turner Davide Zannoni

Pseudomonas pseudoalcaligenes KF707 is a soil bacterium which is known for its capacity to aerobically degrade harmful organic compounds such as polychlorinated biphenyls (PCBs) using biphenyl as co-metabolite. Here we provide the first genetic and functional analysis of the KF707 respiratory terminal oxidases in cells grown with two different carbon sources: glucose and biphenyl. We identified...

Journal: :Journal of bacteriology 2003
Takahito Watanabe Hidehiko Fujihara Kensuke Furukawa

Pseudomonas pseudoalcaligenes KF707 possesses a biphenyl-catabolic (bph) gene cluster consisting of bphR1A1A2-(orf3)-bphA3A4BCX0X1X2X3D. The bphR1 (formerly orf0) gene product, which belongs to the GntR family, is a positive regulator for itself and bphX0X1X2X3D. Further analysis in this study revealed that a second regulator belonging to the LysR family (designated bphR2) is involved in the re...

Journal: :Applied and environmental microbiology 2009
Valentina Tremaroli Matthew L Workentine Aalim M Weljie Hans J Vogel Howard Ceri Carlo Viti Enrico Tatti Ping Zhang Alexander P Hynes Raymond J Turner Davide Zannoni

Pseudomonas pseudoalcaligenes KF707 is naturally resistant to the toxic metalloid tellurite, but the mechanisms of resistance are not known. In this study we report the isolation of a KF707 mutant (T5) with hyperresistance to tellurite. In order to characterize the bacterial response and the pathways leading to tolerance, we utilized Phenotype MicroArray technology (Biolog) and a metabolomic te...

2013
Verónica Hernández-Sánchez Elke Lang Regina-Michaela Wittich

Burkholderia xenovorans LB400, Cupriavidus necator H850, and Pseudomonas pseudoalcaligenes KF707 are bacterial strains able to mineralize biphenyl and to co-oxidize many of its halogenated derivatives (PCBs). Only strain LB400 also mineralizes a few mono- and dichlorobiphenyls, due to the presence of a functioning chlorocatechol pathway. Here, we used a Tn5-based minitransposon shuttle system t...

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