five different media with two concentrations and kinds of carbohydrates and peptone treatments were examined for their effectiveness in promoting asymbiotic seed germination of epipactis  veratrifolia. results revealed significant differences between media on seed germination percentage and protocorm growth and fast medium has the best effect on both. carbohydrate treatments had significant eff...

Auxin flows in a polar manner to target tissues and exert its morphogenic effect. Preventing auxin movement, with polar auxin transport (PAT) inhibitors, or increasing auxin levels in tissues through exogenous application can provide a means for assessing the importance of appropriate tissue distribution and concentration of this hormone during development. The formulation of culture media for ...

in this study, in vitro non-symbiotic germination of self pollinated seeds, protocorms survival percentage and protocorms growth of phalaenopsis ‘kyoto’ were investigated. for this purpose, five different modified media include vasin and went (vw), murashing and skoog (ms), ½ ms, knudson c (kc), fast that supplemented with two g/l peptone, 15 percent sterile coconut water or two g/l peptone tog...

This paper investigates an optimization approach to determining the placement and timing of habitat protection for the western prairie fringed orchid. This plant’s population dynamics are complex, creating a challenging optimization problem. The sensitivity of the orchid to random climate conditions is handled probabilistically. The plant’s seed, protocorm and above-ground stages are all accoun...

Immature embryos of Cymbidium iridioides were successfully cultured on five different media with various supplements and maintained in three different light conditions. Within three week of culture, embryos formed nodular structures. Cultures under full light condition formed green protocorm-like bodies while, other light conditions failed to support optimum germination. Immature embryos of 10 ...

The ca. 400-million-year-old Rhynie chert biota represents a benchmark for studies of early terrestrial ecosystems. The exquisite preservation of the organisms documents an ancient biodiversity that also includes various levels of biological interaction. Absent from the picture until recently has been detailed information about the development of the gametophyte phase and the alternation of gen...

A comparative study of in vitro seed germination of two endangered orchid species, viz. Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall., was carried out on Murashige and Skoog’s (MS) medium, supplemented with different concentrations and combination of 6-benzylaminopurine (BAP) and á-Naphthalene acetic acid (NAA). The hormone-free MS medium and MS medium supplemented with va...

A critical step in the development of Agrobacterium tumefaciens-mediated transformation in orchid genus is the establishment of optimal conditions for T-DNA gene delivery into protocorm-like bodies from which the whole plantlets can be regenerated. As a first step in the development of a successful Agrobacterium tumefaciens-mediated transformation for Dendrobium savin white, factors influencing...

Gametophytic selection was examined as a breeding tool in developing Phalaenopsis hybrids that are more cool or warm temperature tolerant. Two hybrid Phalaenopsis, P. (Taisoco Windian × Sogo Yukidian) by P. hybrid unknown, were reciprocally crosspollinated and exposed to 14°C/9°C for 7 days as a cold pollination treatment. Plants were pollinated again and exposed to 30°C/25°C for 3 days for the...