the date palm fruit stalk borer is one of the most important pests of date palm in the world. biochemical properties of digestive proteases in oryctes elegans prell larvae were investigated in this research and optimal total proteolytic and trypsin activities were obtained at ph 9.0 and 11.0, respectively. activity staining of protease on sds-page showed one isoform. also, zymogram pattern of t...

the aim of this study was to determine the inhibitory effects of feed ingredients on protease activities of argyrosomus regius larvae using in vitro techniques. a. regius larvae fed on a commercial feeding procedure were sampled thirteen times, during the sampling period (from 3 to 32 days after hatching (dah)). the differences observed in protease activities of meagre larvae during the samplin...

the protease enzyme breaks down protein into amino acids as its constructors. protease immobilization onto appropriate support materials plays an essential role in various fields of technology including the food and detergent industries. accordingly, improvement of protease immobilization has been highly regarded due to its applications in bio catalysis. alginate and chitosan are natural polysa...

Penicilliurn expamum grown in a medium with rice husk as a carbon source produced an extracellular protease. The protease enzyme was isolated from culture broth by fractionation with acetone and column chromatography on Sephadex G- 100 and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery of 14%. The purified protease was homogenous on SDS polyacrylarnide disc ge...

conclusions in this study, we isolated b. licheniformis producing an organic solvent-stable protease from oil-contaminated soil. the protease was stable and active in various organic solvents. by purification, the protease could be used as a biocatalyst for organic solvent-based enzymatic synthesis. results according to 16srdna sequencing, strain isa9 was identified as a new strain of bacillus ...

htlv-1 and hiv-1 are two major causes for severe t-cell leukemia disease and acquired immune deficiency syndrome (aids). htlv-1 protease, a member of aspartic acid protease family, plays important roles in maturation during virus replication cycle. the impairment of these proteases results in uninfectious htlv-1virions.similar to hiv-1protease deliberate mutations that confer drug resistance on...

Background: Alkaline proteases is the important group of enzymes having numerous industrial applications including dairy food formulations. Objectives: The current study deals with the purification and characterization of an alkaline serine protease produced by Geotrichum candidum QAUGC01, isolated from indigenous fermented milk product, Dahi.<br...

background: fasciola hepatica is one of the most important helminthes parasites and triclabendazole (tcbz) is routinely used for treatment of infected people and animals. secreted protease enzymes by the f. hepatica plays a critical role in the inva-sion, migration, nutrition and the survival of parasite and are key targets for novel drugs and vaccines. the aim of study was to determine the pro...

background: as a drug target and an antigenic agent, hiv-1 protease (hiv-1 pr) is at the center of attention for designing anti-aids inhibitors and diagnostic tests. in previous studies, the production of the recombinant protease has been faced with several difficulties; therefore, the aims of this study were the easy production, purification of the soluble form of protease in e. coli and inves...

human immunodeficiency virus type 1 (hiv-1) protease inhibitors comprise an important class of drugs used in hiv treatments. however, mutations of protease genes accelerated by low fidelity of reverse transcriptase yield drug resistant mutants of reduced affinities for the inhibitors. this problem is considered to be a serious barrier against hiv treatment for the foreseeable future. in this st...