Background: Gaucher disease is an autosomal recessive inherited lysosomal storage disorder that affects many of the body's organs and tissues by defective function of the catabolic enzyme β-glucocerebrosidase. Gene therapy is one of the efficient ways for treatment of this disease. Due to the lack of appropriate animal models, in the field of gene therapy little progress has been done.Mate...

the present study was performed to evaluate the analgesic effect of morphine microinjection into the cuneiformis nucleus (cnf) and the effect of inactivation of this area by lidocaine on pain modulation. rats were anaesthetized by thiopental (45-60 mg/kg/i.p.) and placed in a stereotaxic instrument, and then a guide cannula was implanted just one mm above the cnf. following surgery and recovery...

Virus spillover from managed to unmanaged bees and vice versa may be one mechanism driving colony losses of the former declines latter. There is clear evidence that ubiquitous Deformed wing virus (DWV) a major driver honey bee ( Apis mellifera ) mortality. Although DWV has been detected in solitary Osmia bicornis , data on infectivity virulence are scarce. Here, we used microinjection investiga...

The present study was performed to evaluate the analgesic effect of morphine microinjection into the cuneiformis nucleus (CnF) and the effect of inactivation of this area by lidocaine on pain modulation. Rats were anaesthetized by thiopental (45-60 mg/kg/i.p.) and placed in a stereotaxic instrument, and then a guide cannula was implanted just one mm above the CnF. Following surgery and recovery...

The melanocortin-4 receptor (MC4R) plays a critical role in homeostasis and the regulation of body weight. Polymorphisms mc4r gene have been discovered linked to growth, carcass composition, meat quality traits. Therefore, we used CRISPR-Cas9 system target most important freshwater aquaculture species USA, channel catfish, Ictalurus punctatus. Guide RNAs were designed direct Cas9 coding sequenc...

introduction: we previously shown that microinjection of glutamate into the hdb of rat elicited cardiovascular depressive responses. microinjection of ap5 (an nmda receptor antagonist, 2.5 mm, 50 nl) and cnqx (an ampa receptor antagonist, 1 mm, 50 nl) caused no significant changes in the blood pressure and heart rate. microinjection of bicuculline (bmi: a gabaa receptor antagonist, 1 mm, 50 nl)...